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N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION

Colorectal cancer is the third most common cancer worldwide with an annual incidence of ∼1 million cases and an annual mortality rate of ∼655,000 individuals. There is an urgent need for identifying novel targets to develop more sensitive, reliable, and specific tests for early stage detection of co...

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Autores principales: Balog, Crina I. A., Stavenhagen, Kathrin, Fung, Wesley L. J., Koeleman, Carolien A., McDonnell, Liam A., Verhoeven, Aswin, Mesker, Wilma E., Tollenaar, Rob A. E. M., Deelder, André M., Wuhrer, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434767/
https://www.ncbi.nlm.nih.gov/pubmed/22573871
http://dx.doi.org/10.1074/mcp.M111.011601
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author Balog, Crina I. A.
Stavenhagen, Kathrin
Fung, Wesley L. J.
Koeleman, Carolien A.
McDonnell, Liam A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
author_facet Balog, Crina I. A.
Stavenhagen, Kathrin
Fung, Wesley L. J.
Koeleman, Carolien A.
McDonnell, Liam A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
author_sort Balog, Crina I. A.
collection PubMed
description Colorectal cancer is the third most common cancer worldwide with an annual incidence of ∼1 million cases and an annual mortality rate of ∼655,000 individuals. There is an urgent need for identifying novel targets to develop more sensitive, reliable, and specific tests for early stage detection of colon cancer. Post-translational modifications are known to play an important role in cancer progression and immune surveillance of tumors. In the present study, we compared the N-glycan profiles from 13 colorectal cancer tumor tissues and corresponding control colon tissues. The N-glycans were enzymatically released, purified, and labeled with 2-aminobenzoic acid. Aliquots were profiled by hydrophilic interaction liquid chromatography (HILIC-HPLC) with fluorescence detection and by negative mode MALDI-TOF-MS. Using partial least squares discriminant analysis to investigate the N-glycosylation changes in colorectal cancer, an excellent separation and prediction ability were observed for both HILIC-HPLC and MALDI-TOF-MS data. For structure elucidation, information from positive mode ESI-ion trap-MS/MS and negative mode MALDI-TOF/TOF-MS was combined. Among the features with a high separation power, structures containing a bisecting GlcNAc were found to be decreased in the tumor, whereas sulfated glycans, paucimannosidic glycans, and glycans containing a sialylated Lewis type epitope were shown to be increased in tumor tissues. In addition, core-fucosylated high mannose N-glycans were detected in tumor samples. In conclusion, the combination of HILIC and MALDI-TOF-MS profiling of N-glycans with multivariate statistical analysis demonstrated its potential for identifying N-glycosylation changes in colorectal cancer tissues and provided new leads that might be used as candidate biomarkers.
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spelling pubmed-34347672012-09-11 N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION Balog, Crina I. A. Stavenhagen, Kathrin Fung, Wesley L. J. Koeleman, Carolien A. McDonnell, Liam A. Verhoeven, Aswin Mesker, Wilma E. Tollenaar, Rob A. E. M. Deelder, André M. Wuhrer, Manfred Mol Cell Proteomics Research Colorectal cancer is the third most common cancer worldwide with an annual incidence of ∼1 million cases and an annual mortality rate of ∼655,000 individuals. There is an urgent need for identifying novel targets to develop more sensitive, reliable, and specific tests for early stage detection of colon cancer. Post-translational modifications are known to play an important role in cancer progression and immune surveillance of tumors. In the present study, we compared the N-glycan profiles from 13 colorectal cancer tumor tissues and corresponding control colon tissues. The N-glycans were enzymatically released, purified, and labeled with 2-aminobenzoic acid. Aliquots were profiled by hydrophilic interaction liquid chromatography (HILIC-HPLC) with fluorescence detection and by negative mode MALDI-TOF-MS. Using partial least squares discriminant analysis to investigate the N-glycosylation changes in colorectal cancer, an excellent separation and prediction ability were observed for both HILIC-HPLC and MALDI-TOF-MS data. For structure elucidation, information from positive mode ESI-ion trap-MS/MS and negative mode MALDI-TOF/TOF-MS was combined. Among the features with a high separation power, structures containing a bisecting GlcNAc were found to be decreased in the tumor, whereas sulfated glycans, paucimannosidic glycans, and glycans containing a sialylated Lewis type epitope were shown to be increased in tumor tissues. In addition, core-fucosylated high mannose N-glycans were detected in tumor samples. In conclusion, the combination of HILIC and MALDI-TOF-MS profiling of N-glycans with multivariate statistical analysis demonstrated its potential for identifying N-glycosylation changes in colorectal cancer tissues and provided new leads that might be used as candidate biomarkers. The American Society for Biochemistry and Molecular Biology 2012-09 2012-05-09 /pmc/articles/PMC3434767/ /pubmed/22573871 http://dx.doi.org/10.1074/mcp.M111.011601 Text en © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Research
Balog, Crina I. A.
Stavenhagen, Kathrin
Fung, Wesley L. J.
Koeleman, Carolien A.
McDonnell, Liam A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title_full N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title_fullStr N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title_full_unstemmed N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title_short N-glycosylation of Colorectal Cancer Tissues: A LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY-BASED INVESTIGATION
title_sort n-glycosylation of colorectal cancer tissues: a liquid chromatography and mass spectrometry-based investigation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434767/
https://www.ncbi.nlm.nih.gov/pubmed/22573871
http://dx.doi.org/10.1074/mcp.M111.011601
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