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SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity
Phosphorylation or SUMOylation of the kainate receptor (KAR) subunit GluK2 have both individually been shown to regulate KAR surface expression. However, it is unknown if phosphorylation and SUMOylation of GluK2 are important for activity-dependent KAR synaptic plasticity. Here, we show that PKC-med...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435142/ https://www.ncbi.nlm.nih.gov/pubmed/22522402 http://dx.doi.org/10.1038/nn.3089 |
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author | Chamberlain, Sophie E L González-González, Inmaculada M Wilkinson, Kevin A Konopacki, Filip A Kantamneni, Sriharsha Henley, Jeremy M Mellor, Jack R |
author_facet | Chamberlain, Sophie E L González-González, Inmaculada M Wilkinson, Kevin A Konopacki, Filip A Kantamneni, Sriharsha Henley, Jeremy M Mellor, Jack R |
author_sort | Chamberlain, Sophie E L |
collection | PubMed |
description | Phosphorylation or SUMOylation of the kainate receptor (KAR) subunit GluK2 have both individually been shown to regulate KAR surface expression. However, it is unknown if phosphorylation and SUMOylation of GluK2 are important for activity-dependent KAR synaptic plasticity. Here, we show that PKC-mediated phosphorylation of GluK2 at serine 868 promotes GluK2 SUMOylation at lysine 886 and that both these events are necessary for the internalization of GluK2 containing KARs that occurs during long-term depression of KAR-mediated synaptic transmission at rat hippocampal mossy fiber synapses. Conversely, phosphorylation of GluK2 at serine 868 in the absence of SUMOylation leads to an increase in KAR surface expression by facilitating receptor recycling between endosomal compartments and the plasma membrane. Thus, we describe a role for the dynamic control of synaptic SUMOylation in the regulation of KAR synaptic transmission and plasticity. |
format | Online Article Text |
id | pubmed-3435142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-34351422012-12-01 SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity Chamberlain, Sophie E L González-González, Inmaculada M Wilkinson, Kevin A Konopacki, Filip A Kantamneni, Sriharsha Henley, Jeremy M Mellor, Jack R Nat Neurosci Article Phosphorylation or SUMOylation of the kainate receptor (KAR) subunit GluK2 have both individually been shown to regulate KAR surface expression. However, it is unknown if phosphorylation and SUMOylation of GluK2 are important for activity-dependent KAR synaptic plasticity. Here, we show that PKC-mediated phosphorylation of GluK2 at serine 868 promotes GluK2 SUMOylation at lysine 886 and that both these events are necessary for the internalization of GluK2 containing KARs that occurs during long-term depression of KAR-mediated synaptic transmission at rat hippocampal mossy fiber synapses. Conversely, phosphorylation of GluK2 at serine 868 in the absence of SUMOylation leads to an increase in KAR surface expression by facilitating receptor recycling between endosomal compartments and the plasma membrane. Thus, we describe a role for the dynamic control of synaptic SUMOylation in the regulation of KAR synaptic transmission and plasticity. 2012-06 /pmc/articles/PMC3435142/ /pubmed/22522402 http://dx.doi.org/10.1038/nn.3089 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Chamberlain, Sophie E L González-González, Inmaculada M Wilkinson, Kevin A Konopacki, Filip A Kantamneni, Sriharsha Henley, Jeremy M Mellor, Jack R SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title | SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title_full | SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title_fullStr | SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title_full_unstemmed | SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title_short | SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity |
title_sort | sumoylation and phosphorylation of gluk2 regulate kainate receptor trafficking and synaptic plasticity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435142/ https://www.ncbi.nlm.nih.gov/pubmed/22522402 http://dx.doi.org/10.1038/nn.3089 |
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