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DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children

BACKGROUND: Allergic inflammation is commonly observed in a number of conditions that are associated with atopy including asthma, eczema and rhinitis. However, the genetic, environmental or epigenetic factors involved in these conditions are likely to be different. Epigenetic modifications, such as...

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Autores principales: Stefanowicz, Dorota, Hackett, Tillie-Louise, Garmaroudi, Farshid S., Günther, Oliver P., Neumann, Sarah, Sutanto, Erika N., Ling, Kak-Ming, Kobor, Michael S., Kicic, Anthony, Stick, Stephen M., Paré, Peter D., Knight, Darryl A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435400/
https://www.ncbi.nlm.nih.gov/pubmed/22970180
http://dx.doi.org/10.1371/journal.pone.0044213
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author Stefanowicz, Dorota
Hackett, Tillie-Louise
Garmaroudi, Farshid S.
Günther, Oliver P.
Neumann, Sarah
Sutanto, Erika N.
Ling, Kak-Ming
Kobor, Michael S.
Kicic, Anthony
Stick, Stephen M.
Paré, Peter D.
Knight, Darryl A.
author_facet Stefanowicz, Dorota
Hackett, Tillie-Louise
Garmaroudi, Farshid S.
Günther, Oliver P.
Neumann, Sarah
Sutanto, Erika N.
Ling, Kak-Ming
Kobor, Michael S.
Kicic, Anthony
Stick, Stephen M.
Paré, Peter D.
Knight, Darryl A.
author_sort Stefanowicz, Dorota
collection PubMed
description BACKGROUND: Allergic inflammation is commonly observed in a number of conditions that are associated with atopy including asthma, eczema and rhinitis. However, the genetic, environmental or epigenetic factors involved in these conditions are likely to be different. Epigenetic modifications, such as DNA methylation, can be influenced by the environment and result in changes to gene expression. OBJECTIVES: To characterize the DNA methylation pattern of airway epithelial cells (AECs) compared to peripheral blood mononuclear cells (PBMCs) and to discern differences in methylation within each cell type amongst healthy, atopic and asthmatic subjects. METHODS: PBMCs and AECs from bronchial brushings were obtained from children undergoing elective surgery for non-respiratory conditions. The children were categorized as atopic, atopic asthmatic, non-atopic asthmatic or healthy controls. Extracted DNA was bisulfite treated and 1505 CpG loci across 807 genes were analyzed using the Illumina GoldenGate Methylation Cancer Panel I. Gene expression for a subset of genes was performed using RT-PCR. RESULTS: We demonstrate a signature set of CpG sites that are differentially methylated in AECs as compared to PBMCs regardless of disease phenotype. Of these, 13 CpG sites were specific to healthy controls, 8 sites were only found in atopics, and 6 CpGs were unique to asthmatics. We found no differences in the methylation status of PBMCs between disease phenotypes. In AECs derived from asthmatics compared to atopics, 8 differentially methylated sites were identified including CpGs in STAT5A and CRIP1. We demonstrate STAT5A gene expression is decreased whereas CRIP1 gene expression is elevated in the AECs from asthmatic compared to both healthy and atopic subjects. DISCUSSION: We characterized a cell specific DNA methylation signature for AECs compared to PBMCs regardless of asthmatic or atopic status. Our data highlight the importance of understanding DNA methylation in the epithelium when studying the epithelial contribution to asthma.
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spelling pubmed-34354002012-09-11 DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children Stefanowicz, Dorota Hackett, Tillie-Louise Garmaroudi, Farshid S. Günther, Oliver P. Neumann, Sarah Sutanto, Erika N. Ling, Kak-Ming Kobor, Michael S. Kicic, Anthony Stick, Stephen M. Paré, Peter D. Knight, Darryl A. PLoS One Research Article BACKGROUND: Allergic inflammation is commonly observed in a number of conditions that are associated with atopy including asthma, eczema and rhinitis. However, the genetic, environmental or epigenetic factors involved in these conditions are likely to be different. Epigenetic modifications, such as DNA methylation, can be influenced by the environment and result in changes to gene expression. OBJECTIVES: To characterize the DNA methylation pattern of airway epithelial cells (AECs) compared to peripheral blood mononuclear cells (PBMCs) and to discern differences in methylation within each cell type amongst healthy, atopic and asthmatic subjects. METHODS: PBMCs and AECs from bronchial brushings were obtained from children undergoing elective surgery for non-respiratory conditions. The children were categorized as atopic, atopic asthmatic, non-atopic asthmatic or healthy controls. Extracted DNA was bisulfite treated and 1505 CpG loci across 807 genes were analyzed using the Illumina GoldenGate Methylation Cancer Panel I. Gene expression for a subset of genes was performed using RT-PCR. RESULTS: We demonstrate a signature set of CpG sites that are differentially methylated in AECs as compared to PBMCs regardless of disease phenotype. Of these, 13 CpG sites were specific to healthy controls, 8 sites were only found in atopics, and 6 CpGs were unique to asthmatics. We found no differences in the methylation status of PBMCs between disease phenotypes. In AECs derived from asthmatics compared to atopics, 8 differentially methylated sites were identified including CpGs in STAT5A and CRIP1. We demonstrate STAT5A gene expression is decreased whereas CRIP1 gene expression is elevated in the AECs from asthmatic compared to both healthy and atopic subjects. DISCUSSION: We characterized a cell specific DNA methylation signature for AECs compared to PBMCs regardless of asthmatic or atopic status. Our data highlight the importance of understanding DNA methylation in the epithelium when studying the epithelial contribution to asthma. Public Library of Science 2012-09-06 /pmc/articles/PMC3435400/ /pubmed/22970180 http://dx.doi.org/10.1371/journal.pone.0044213 Text en © 2012 Stefanowicz et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Stefanowicz, Dorota
Hackett, Tillie-Louise
Garmaroudi, Farshid S.
Günther, Oliver P.
Neumann, Sarah
Sutanto, Erika N.
Ling, Kak-Ming
Kobor, Michael S.
Kicic, Anthony
Stick, Stephen M.
Paré, Peter D.
Knight, Darryl A.
DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title_full DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title_fullStr DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title_full_unstemmed DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title_short DNA Methylation Profiles of Airway Epithelial Cells and PBMCs from Healthy, Atopic and Asthmatic Children
title_sort dna methylation profiles of airway epithelial cells and pbmcs from healthy, atopic and asthmatic children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435400/
https://www.ncbi.nlm.nih.gov/pubmed/22970180
http://dx.doi.org/10.1371/journal.pone.0044213
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