Detection of miRNA in Cell Cultures by Using Microchip Electrophoresis with a Fluorescence-Labeled Riboprobe

The analysis of a microRNA (miRNA), miR-222 isolated from the PC12 cell line, was performed by use of the ribonuclease (RNase) protection assay, cyanine 5 (Cy5)-labeled miR-222 riboprobe, and a Hitachi SV1210 microchip electrophoresis system, which can be used to evaluate the integrity of total RNA....

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Detalles Bibliográficos
Autores principales: Yamamura, Shohei, Yatsushiro, Shouki, Yamaguchi, Yuka, Abe, Kaori, Shinohara, Yasuo, Kataoka, Masatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435990/
https://www.ncbi.nlm.nih.gov/pubmed/22969361
http://dx.doi.org/10.3390/s120607576

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435990/
https://www.ncbi.nlm.nih.gov/pubmed/22969361
http://dx.doi.org/10.3390/s120607576

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