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Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes

The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to...

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Autores principales: Kang, Myoung-Goo, Nuriya, Mutsuo, Guo, Yurong, Martindale, Kevin D., Lee, Daniel Z., Huganir, Richard L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436506/
https://www.ncbi.nlm.nih.gov/pubmed/22753414
http://dx.doi.org/10.1074/jbc.M111.336644
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author Kang, Myoung-Goo
Nuriya, Mutsuo
Guo, Yurong
Martindale, Kevin D.
Lee, Daniel Z.
Huganir, Richard L.
author_facet Kang, Myoung-Goo
Nuriya, Mutsuo
Guo, Yurong
Martindale, Kevin D.
Lee, Daniel Z.
Huganir, Richard L.
author_sort Kang, Myoung-Goo
collection PubMed
description The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain. AMPA-R complexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.
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spelling pubmed-34365062012-09-10 Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes Kang, Myoung-Goo Nuriya, Mutsuo Guo, Yurong Martindale, Kevin D. Lee, Daniel Z. Huganir, Richard L. J Biol Chem Neurobiology The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain. AMPA-R complexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity. American Society for Biochemistry and Molecular Biology 2012-08-17 2012-06-29 /pmc/articles/PMC3436506/ /pubmed/22753414 http://dx.doi.org/10.1074/jbc.M111.336644 Text en © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Neurobiology
Kang, Myoung-Goo
Nuriya, Mutsuo
Guo, Yurong
Martindale, Kevin D.
Lee, Daniel Z.
Huganir, Richard L.
Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title_full Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title_fullStr Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title_full_unstemmed Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title_short Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes
title_sort proteomic analysis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes
topic Neurobiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436506/
https://www.ncbi.nlm.nih.gov/pubmed/22753414
http://dx.doi.org/10.1074/jbc.M111.336644
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