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In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens

BACKGROUND: A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic c...

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Autores principales: Hu, Yan, Zhang, Rui, Zhang, Yanhong, Li, Jing, Grossmann, Roland, Zhao, Ruqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436634/
https://www.ncbi.nlm.nih.gov/pubmed/22958551
http://dx.doi.org/10.1186/2049-1891-3-16
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author Hu, Yan
Zhang, Rui
Zhang, Yanhong
Li, Jing
Grossmann, Roland
Zhao, Ruqian
author_facet Hu, Yan
Zhang, Rui
Zhang, Yanhong
Li, Jing
Grossmann, Roland
Zhao, Ruqian
author_sort Hu, Yan
collection PubMed
description BACKGROUND: A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic concentrations of metabolites and hormones, as well as on the expression of genes involved in hepatic lipid metabolism and the predicted microRNAs (miRNAs) targeting the affected genes. To this end we injected fertile eggs with either 0.5 μg of recombinant murine leptin or vehicle (PBS) before incubation. RESULTS: Prenatally leptin-exposed chicks showed lower hatch weight, but higher liver weight relative to the body weight, compared to the control group. In ovo leptin treatment increased the hepatic content and serum concentration of leptin in newly hatched chickens. The hepatic contents of triglycerides (TG) and total cholesterol (Tch) were decreased, whereas the serum levels of TG, Tch and apolipoprotein B (ApoB) were increased. The hepatic mRNA expression of sterol regulator element binding protein 1 (SREBP-1c), SREBP-2, hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) and cholesterol 7α-hydroxylase 1 (CYP7A1) was significantly up-regulated, as was the protein content of both SREBP-1c and SREBP-2 in hepatic nuclear extracts of leptin-treated chickens. Moreover, out of 12 miRNAs targeting SREBP-1c and/or HMGCR, five were significantly up-regulated in liver of leptin-treated chicks, including gga-miR-200b and gga-miR-429, which target both SREBP-1c and HMGCR. CONCLUSIONS: These results suggest that leptin in ovo decreases hatch weight, and modifies hepatic leptin secretion and lipid metabolism in newly hatched broiler chickens, possibly via microRNA-mediated gene regulation.
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spelling pubmed-34366342012-09-08 In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens Hu, Yan Zhang, Rui Zhang, Yanhong Li, Jing Grossmann, Roland Zhao, Ruqian J Anim Sci Biotechnol Research BACKGROUND: A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic concentrations of metabolites and hormones, as well as on the expression of genes involved in hepatic lipid metabolism and the predicted microRNAs (miRNAs) targeting the affected genes. To this end we injected fertile eggs with either 0.5 μg of recombinant murine leptin or vehicle (PBS) before incubation. RESULTS: Prenatally leptin-exposed chicks showed lower hatch weight, but higher liver weight relative to the body weight, compared to the control group. In ovo leptin treatment increased the hepatic content and serum concentration of leptin in newly hatched chickens. The hepatic contents of triglycerides (TG) and total cholesterol (Tch) were decreased, whereas the serum levels of TG, Tch and apolipoprotein B (ApoB) were increased. The hepatic mRNA expression of sterol regulator element binding protein 1 (SREBP-1c), SREBP-2, hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) and cholesterol 7α-hydroxylase 1 (CYP7A1) was significantly up-regulated, as was the protein content of both SREBP-1c and SREBP-2 in hepatic nuclear extracts of leptin-treated chickens. Moreover, out of 12 miRNAs targeting SREBP-1c and/or HMGCR, five were significantly up-regulated in liver of leptin-treated chicks, including gga-miR-200b and gga-miR-429, which target both SREBP-1c and HMGCR. CONCLUSIONS: These results suggest that leptin in ovo decreases hatch weight, and modifies hepatic leptin secretion and lipid metabolism in newly hatched broiler chickens, possibly via microRNA-mediated gene regulation. BioMed Central 2012-06-01 /pmc/articles/PMC3436634/ /pubmed/22958551 http://dx.doi.org/10.1186/2049-1891-3-16 Text en Copyright ©2012 Hu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hu, Yan
Zhang, Rui
Zhang, Yanhong
Li, Jing
Grossmann, Roland
Zhao, Ruqian
In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title_full In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title_fullStr In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title_full_unstemmed In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title_short In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens
title_sort in ovo leptin administration affects hepatic lipid metabolism and microrna expression in newly hatched broiler chickens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436634/
https://www.ncbi.nlm.nih.gov/pubmed/22958551
http://dx.doi.org/10.1186/2049-1891-3-16
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