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ProteinTracker: an application for managing protein production and purification

BACKGROUND: Laboratories that produce protein reagents for research and development face the challenge of deciding whether to track batch-related data using simple file based storage mechanisms (e.g. spreadsheets and notebooks), or commit the time and effort to install, configure and maintain a more...

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Autores principales: Ponko, Stefan C, Bienvenue, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436699/
https://www.ncbi.nlm.nih.gov/pubmed/22574679
http://dx.doi.org/10.1186/1756-0500-5-224
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author Ponko, Stefan C
Bienvenue, David
author_facet Ponko, Stefan C
Bienvenue, David
author_sort Ponko, Stefan C
collection PubMed
description BACKGROUND: Laboratories that produce protein reagents for research and development face the challenge of deciding whether to track batch-related data using simple file based storage mechanisms (e.g. spreadsheets and notebooks), or commit the time and effort to install, configure and maintain a more complex laboratory information management system (LIMS). Managing reagent data stored in files is challenging because files are often copied, moved, and reformatted. Furthermore, there is no simple way to query the data if/when questions arise. Commercial LIMS often include additional modules that may be paid for but not actually used, and often require software expertise to truly customize them for a given environment. FINDINGS: This web-application allows small to medium-sized protein production groups to track data related to plasmid DNA, conditioned media samples (supes), cell lines used for expression, and purified protein information, including method of purification and quality control results. In addition, a request system was added that includes a means of prioritizing requests to help manage the high demand of protein production resources at most organizations. ProteinTracker makes extensive use of existing open-source libraries and is designed to track essential data related to the production and purification of proteins. CONCLUSIONS: ProteinTracker is an open-source web-based application that provides organizations with the ability to track key data involved in the production and purification of proteins and may be modified to meet the specific needs of an organization. The source code and database setup script can be downloaded from http://sourceforge.net/projects/proteintracker. This site also contains installation instructions and a user guide. A demonstration version of the application can be viewed at http://www.proteintracker.org.
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spelling pubmed-34366992012-09-08 ProteinTracker: an application for managing protein production and purification Ponko, Stefan C Bienvenue, David BMC Res Notes Technical Note BACKGROUND: Laboratories that produce protein reagents for research and development face the challenge of deciding whether to track batch-related data using simple file based storage mechanisms (e.g. spreadsheets and notebooks), or commit the time and effort to install, configure and maintain a more complex laboratory information management system (LIMS). Managing reagent data stored in files is challenging because files are often copied, moved, and reformatted. Furthermore, there is no simple way to query the data if/when questions arise. Commercial LIMS often include additional modules that may be paid for but not actually used, and often require software expertise to truly customize them for a given environment. FINDINGS: This web-application allows small to medium-sized protein production groups to track data related to plasmid DNA, conditioned media samples (supes), cell lines used for expression, and purified protein information, including method of purification and quality control results. In addition, a request system was added that includes a means of prioritizing requests to help manage the high demand of protein production resources at most organizations. ProteinTracker makes extensive use of existing open-source libraries and is designed to track essential data related to the production and purification of proteins. CONCLUSIONS: ProteinTracker is an open-source web-based application that provides organizations with the ability to track key data involved in the production and purification of proteins and may be modified to meet the specific needs of an organization. The source code and database setup script can be downloaded from http://sourceforge.net/projects/proteintracker. This site also contains installation instructions and a user guide. A demonstration version of the application can be viewed at http://www.proteintracker.org. BioMed Central 2012-05-10 /pmc/articles/PMC3436699/ /pubmed/22574679 http://dx.doi.org/10.1186/1756-0500-5-224 Text en Copyright ©2012 Ponko and Bienvenue; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Ponko, Stefan C
Bienvenue, David
ProteinTracker: an application for managing protein production and purification
title ProteinTracker: an application for managing protein production and purification
title_full ProteinTracker: an application for managing protein production and purification
title_fullStr ProteinTracker: an application for managing protein production and purification
title_full_unstemmed ProteinTracker: an application for managing protein production and purification
title_short ProteinTracker: an application for managing protein production and purification
title_sort proteintracker: an application for managing protein production and purification
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436699/
https://www.ncbi.nlm.nih.gov/pubmed/22574679
http://dx.doi.org/10.1186/1756-0500-5-224
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