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Proteomic Analyses of the Vitreous Humour

The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine s...

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Detalles Bibliográficos
Autores principales: Angi, Martina, Kalirai, Helen, Coupland, Sarah E., Damato, Bertil E., Semeraro, Francesco, Romano, Mario R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437669/
https://www.ncbi.nlm.nih.gov/pubmed/22973072
http://dx.doi.org/10.1155/2012/148039
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author Angi, Martina
Kalirai, Helen
Coupland, Sarah E.
Damato, Bertil E.
Semeraro, Francesco
Romano, Mario R.
author_facet Angi, Martina
Kalirai, Helen
Coupland, Sarah E.
Damato, Bertil E.
Semeraro, Francesco
Romano, Mario R.
author_sort Angi, Martina
collection PubMed
description The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses.
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spelling pubmed-34376692012-09-12 Proteomic Analyses of the Vitreous Humour Angi, Martina Kalirai, Helen Coupland, Sarah E. Damato, Bertil E. Semeraro, Francesco Romano, Mario R. Mediators Inflamm Review Article The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses. Hindawi Publishing Corporation 2012 2012-08-29 /pmc/articles/PMC3437669/ /pubmed/22973072 http://dx.doi.org/10.1155/2012/148039 Text en Copyright © 2012 Martina Angi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Angi, Martina
Kalirai, Helen
Coupland, Sarah E.
Damato, Bertil E.
Semeraro, Francesco
Romano, Mario R.
Proteomic Analyses of the Vitreous Humour
title Proteomic Analyses of the Vitreous Humour
title_full Proteomic Analyses of the Vitreous Humour
title_fullStr Proteomic Analyses of the Vitreous Humour
title_full_unstemmed Proteomic Analyses of the Vitreous Humour
title_short Proteomic Analyses of the Vitreous Humour
title_sort proteomic analyses of the vitreous humour
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437669/
https://www.ncbi.nlm.nih.gov/pubmed/22973072
http://dx.doi.org/10.1155/2012/148039
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