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Proteomic Analyses of the Vitreous Humour
The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437669/ https://www.ncbi.nlm.nih.gov/pubmed/22973072 http://dx.doi.org/10.1155/2012/148039 |
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author | Angi, Martina Kalirai, Helen Coupland, Sarah E. Damato, Bertil E. Semeraro, Francesco Romano, Mario R. |
author_facet | Angi, Martina Kalirai, Helen Coupland, Sarah E. Damato, Bertil E. Semeraro, Francesco Romano, Mario R. |
author_sort | Angi, Martina |
collection | PubMed |
description | The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses. |
format | Online Article Text |
id | pubmed-3437669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34376692012-09-12 Proteomic Analyses of the Vitreous Humour Angi, Martina Kalirai, Helen Coupland, Sarah E. Damato, Bertil E. Semeraro, Francesco Romano, Mario R. Mediators Inflamm Review Article The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses. Hindawi Publishing Corporation 2012 2012-08-29 /pmc/articles/PMC3437669/ /pubmed/22973072 http://dx.doi.org/10.1155/2012/148039 Text en Copyright © 2012 Martina Angi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Angi, Martina Kalirai, Helen Coupland, Sarah E. Damato, Bertil E. Semeraro, Francesco Romano, Mario R. Proteomic Analyses of the Vitreous Humour |
title | Proteomic Analyses of the Vitreous Humour |
title_full | Proteomic Analyses of the Vitreous Humour |
title_fullStr | Proteomic Analyses of the Vitreous Humour |
title_full_unstemmed | Proteomic Analyses of the Vitreous Humour |
title_short | Proteomic Analyses of the Vitreous Humour |
title_sort | proteomic analyses of the vitreous humour |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437669/ https://www.ncbi.nlm.nih.gov/pubmed/22973072 http://dx.doi.org/10.1155/2012/148039 |
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