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MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood
Exploiting the properties of stem cells by microRNA (miRNA) profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC), the targets corresponding to many of these miRNA have not yet been fully e...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437805/ https://www.ncbi.nlm.nih.gov/pubmed/23344176 http://dx.doi.org/10.1038/mtna.2012.29 |
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author | Mintz, Paul J Sætrom, Pål Reebye, Vikash Lundbæk, Marie B Lao, Kaiqin Rossi, John J Gaensler, Karin ML Kasahara, Noriyuki Nicholls, Joanna P Jensen, Steen Haoudi, Abdelali Emara, Mohamed M Gordon, Myrtle YA Habib, Nagy A |
author_facet | Mintz, Paul J Sætrom, Pål Reebye, Vikash Lundbæk, Marie B Lao, Kaiqin Rossi, John J Gaensler, Karin ML Kasahara, Noriyuki Nicholls, Joanna P Jensen, Steen Haoudi, Abdelali Emara, Mohamed M Gordon, Myrtle YA Habib, Nagy A |
author_sort | Mintz, Paul J |
collection | PubMed |
description | Exploiting the properties of stem cells by microRNA (miRNA) profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC), the targets corresponding to many of these miRNA have not yet been fully elucidated. By miRNA profiling in a subpopulation of CD34+ cells isolated from peripheral blood, we have identified eight clusters of miRNA that were differentially expressed. Further analysis of one of the clusters by bioinformatics revealed that a miRNA, miR-181a*, which is highly expressed in the adherent CD34+ cells, affects the expression levels of Nanog, a stem cell surrogate marker. We show specifically by reporter assay and mutational analysis that miR-181a* targets a seedless 3′ compensatory site in the 3′UTR of Nanog and affects gene expression. We demonstrate that inhibiting miR-181a* upregulates the Nanog expression level, in addition to an increase in alkaline phosphatase activity. Our studies suggest that miR-181a* may be important in controlling the expression level of Nanog in a subpopulation of CD34+ cells. |
format | Online Article Text |
id | pubmed-3437805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-34378052012-09-10 MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood Mintz, Paul J Sætrom, Pål Reebye, Vikash Lundbæk, Marie B Lao, Kaiqin Rossi, John J Gaensler, Karin ML Kasahara, Noriyuki Nicholls, Joanna P Jensen, Steen Haoudi, Abdelali Emara, Mohamed M Gordon, Myrtle YA Habib, Nagy A Mol Ther Nucleic Acids Original Article Exploiting the properties of stem cells by microRNA (miRNA) profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC), the targets corresponding to many of these miRNA have not yet been fully elucidated. By miRNA profiling in a subpopulation of CD34+ cells isolated from peripheral blood, we have identified eight clusters of miRNA that were differentially expressed. Further analysis of one of the clusters by bioinformatics revealed that a miRNA, miR-181a*, which is highly expressed in the adherent CD34+ cells, affects the expression levels of Nanog, a stem cell surrogate marker. We show specifically by reporter assay and mutational analysis that miR-181a* targets a seedless 3′ compensatory site in the 3′UTR of Nanog and affects gene expression. We demonstrate that inhibiting miR-181a* upregulates the Nanog expression level, in addition to an increase in alkaline phosphatase activity. Our studies suggest that miR-181a* may be important in controlling the expression level of Nanog in a subpopulation of CD34+ cells. Nature Publishing Group 2012-08 2012-08-07 /pmc/articles/PMC3437805/ /pubmed/23344176 http://dx.doi.org/10.1038/mtna.2012.29 Text en Copyright © 2012 American Society of Gene & Cell Therapy http://creativecommons.org/licenses/by-nc-nd/3.0/ Molecular Therapy-Nucleic Acids is an open-access journal published by Nature Publishing Group. This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Original Article Mintz, Paul J Sætrom, Pål Reebye, Vikash Lundbæk, Marie B Lao, Kaiqin Rossi, John J Gaensler, Karin ML Kasahara, Noriyuki Nicholls, Joanna P Jensen, Steen Haoudi, Abdelali Emara, Mohamed M Gordon, Myrtle YA Habib, Nagy A MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title | MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title_full | MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title_fullStr | MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title_full_unstemmed | MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title_short | MicroRNA-181a* Targets Nanog in a Subpopulation of CD34(+) Cells Isolated From Peripheral Blood |
title_sort | microrna-181a* targets nanog in a subpopulation of cd34(+) cells isolated from peripheral blood |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437805/ https://www.ncbi.nlm.nih.gov/pubmed/23344176 http://dx.doi.org/10.1038/mtna.2012.29 |
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