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Cell Division Site Placement and Asymmetric Growth in Mycobacteria

Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide as...

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Detalles Bibliográficos
Autores principales: Joyce, Graham, Williams, Kerstin J., Robb, Matthew, Noens, Elke, Tizzano, Barbara, Shahrezaei, Vahid, Robertson, Brian D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438161/
https://www.ncbi.nlm.nih.gov/pubmed/22970255
http://dx.doi.org/10.1371/journal.pone.0044582
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author Joyce, Graham
Williams, Kerstin J.
Robb, Matthew
Noens, Elke
Tizzano, Barbara
Shahrezaei, Vahid
Robertson, Brian D.
author_facet Joyce, Graham
Williams, Kerstin J.
Robb, Matthew
Noens, Elke
Tizzano, Barbara
Shahrezaei, Vahid
Robertson, Brian D.
author_sort Joyce, Graham
collection PubMed
description Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide asymmetrically, and at a wide range of sizes, in contrast to the cell growth and division patterns observed in the model rod-shaped organisms. In this study, we test this hypothesis using live-cell time-lapse imaging of dividing Mycobacterium smegmatis labelled with fluorescent PBP1a, to probe peptidoglycan synthesis and label the cell septum. We demonstrate that the new septum is placed accurately at mid-cell, and that the asymmetric division observed is a result of differential growth from the cell tips, with a more than 2-fold difference in growth rate between fast and slow growing poles. We also show that the division site is not selected at a characteristic cell length, suggesting this is not an important cue during the mycobacterial cell cycle.
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spelling pubmed-34381612012-09-11 Cell Division Site Placement and Asymmetric Growth in Mycobacteria Joyce, Graham Williams, Kerstin J. Robb, Matthew Noens, Elke Tizzano, Barbara Shahrezaei, Vahid Robertson, Brian D. PLoS One Research Article Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide asymmetrically, and at a wide range of sizes, in contrast to the cell growth and division patterns observed in the model rod-shaped organisms. In this study, we test this hypothesis using live-cell time-lapse imaging of dividing Mycobacterium smegmatis labelled with fluorescent PBP1a, to probe peptidoglycan synthesis and label the cell septum. We demonstrate that the new septum is placed accurately at mid-cell, and that the asymmetric division observed is a result of differential growth from the cell tips, with a more than 2-fold difference in growth rate between fast and slow growing poles. We also show that the division site is not selected at a characteristic cell length, suggesting this is not an important cue during the mycobacterial cell cycle. Public Library of Science 2012-09-10 /pmc/articles/PMC3438161/ /pubmed/22970255 http://dx.doi.org/10.1371/journal.pone.0044582 Text en © 2012 Joyce et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Joyce, Graham
Williams, Kerstin J.
Robb, Matthew
Noens, Elke
Tizzano, Barbara
Shahrezaei, Vahid
Robertson, Brian D.
Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title_full Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title_fullStr Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title_full_unstemmed Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title_short Cell Division Site Placement and Asymmetric Growth in Mycobacteria
title_sort cell division site placement and asymmetric growth in mycobacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438161/
https://www.ncbi.nlm.nih.gov/pubmed/22970255
http://dx.doi.org/10.1371/journal.pone.0044582
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