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Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model

OBJECTIVE: Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be e...

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Autores principales: Medeiros, Israel L., Pêgo-Fernandes, Paulo M., Mariani, Alessandro W., Fernandes, Flávio G., Unterpertinger, Fernando V., Canzian, Mauro, Jatene, Fabio B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438253/
https://www.ncbi.nlm.nih.gov/pubmed/23018310
http://dx.doi.org/10.6061/clinics/2012(09)19
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author Medeiros, Israel L.
Pêgo-Fernandes, Paulo M.
Mariani, Alessandro W.
Fernandes, Flávio G.
Unterpertinger, Fernando V.
Canzian, Mauro
Jatene, Fabio B.
author_facet Medeiros, Israel L.
Pêgo-Fernandes, Paulo M.
Mariani, Alessandro W.
Fernandes, Flávio G.
Unterpertinger, Fernando V.
Canzian, Mauro
Jatene, Fabio B.
author_sort Medeiros, Israel L.
collection PubMed
description OBJECTIVE: Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be expected in clinical practice. METHOD: Brain-dead donors whose lungs had been declined by transplantation teams were used. The cases were randomized into two groups. In Group 1, Perfadex® was used for pulmonary preservation, and in Group 2, LPDnac, a solution manufactured in Brazil, was used. An ex vivo lung perfusion system was used, and the lungs were ventilated and perfused after 10 hours of cold ischemia. The extent of ischemic-reperfusion injury was measured using functional and histological parameters. RESULTS: After reperfusion, the mean oxygenation capacity was 405.3 mmHg in Group 1 and 406.0 mmHg in Group 2 (p = 0.98). The mean pulmonary vascular resistance values were 697.6 and 378.3 dyn·s·cm(-5), respectively (p = 0.035). The mean pulmonary compliance was 46.8 cm H(2)O in Group 1 and 49.3 ml/cm H(2)O in Group 2 (p = 0.816). The mean wet/dry weight ratios were 2.06 and 2.02, respectively (p = 0.87). The mean Lung Injury Scores for the biopsy performed after reperfusion were 4.37 and 4.37 in Groups 1 and 2, respectively (p = 1.0), and the apoptotic cell counts were 118.75/mm(2) and 137.50/mm(2), respectively (p = 0.71). CONCLUSION: The locally produced preservation solution proved to be as good as Perfadex®. The clinical use of LPDnac may reduce costs in our centers. Therefore, it is important to develop new models to study lung preservation.
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spelling pubmed-34382532012-09-11 Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model Medeiros, Israel L. Pêgo-Fernandes, Paulo M. Mariani, Alessandro W. Fernandes, Flávio G. Unterpertinger, Fernando V. Canzian, Mauro Jatene, Fabio B. Clinics (Sao Paulo) Basic Research OBJECTIVE: Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be expected in clinical practice. METHOD: Brain-dead donors whose lungs had been declined by transplantation teams were used. The cases were randomized into two groups. In Group 1, Perfadex® was used for pulmonary preservation, and in Group 2, LPDnac, a solution manufactured in Brazil, was used. An ex vivo lung perfusion system was used, and the lungs were ventilated and perfused after 10 hours of cold ischemia. The extent of ischemic-reperfusion injury was measured using functional and histological parameters. RESULTS: After reperfusion, the mean oxygenation capacity was 405.3 mmHg in Group 1 and 406.0 mmHg in Group 2 (p = 0.98). The mean pulmonary vascular resistance values were 697.6 and 378.3 dyn·s·cm(-5), respectively (p = 0.035). The mean pulmonary compliance was 46.8 cm H(2)O in Group 1 and 49.3 ml/cm H(2)O in Group 2 (p = 0.816). The mean wet/dry weight ratios were 2.06 and 2.02, respectively (p = 0.87). The mean Lung Injury Scores for the biopsy performed after reperfusion were 4.37 and 4.37 in Groups 1 and 2, respectively (p = 1.0), and the apoptotic cell counts were 118.75/mm(2) and 137.50/mm(2), respectively (p = 0.71). CONCLUSION: The locally produced preservation solution proved to be as good as Perfadex®. The clinical use of LPDnac may reduce costs in our centers. Therefore, it is important to develop new models to study lung preservation. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2012-09 /pmc/articles/PMC3438253/ /pubmed/23018310 http://dx.doi.org/10.6061/clinics/2012(09)19 Text en Copyright © 2012 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Medeiros, Israel L.
Pêgo-Fernandes, Paulo M.
Mariani, Alessandro W.
Fernandes, Flávio G.
Unterpertinger, Fernando V.
Canzian, Mauro
Jatene, Fabio B.
Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title_full Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title_fullStr Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title_full_unstemmed Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title_short Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
title_sort comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438253/
https://www.ncbi.nlm.nih.gov/pubmed/23018310
http://dx.doi.org/10.6061/clinics/2012(09)19
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