A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in en...

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Detalles Bibliográficos
Autores principales: Russell, Bruce, Suwanarusk, Rossarin, Borlon, Céline, Costa, Fabio T. M., Chu, Cindy S., Rijken, Marcus J., Sriprawat, Kanlaya, Warter, Lucile, Koh, Esther G. L., Malleret, Benoit, Colin, Yves, Bertrand, Olivier, Adams, John H., D'Alessandro, Umberto, Snounou, Georges, Nosten, Francois, Rénia, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Hematology 2011
Materias:
Red Cells, Iron, and Erythropoiesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438884/
https://www.ncbi.nlm.nih.gov/pubmed/21768300
http://dx.doi.org/10.1182/blood-2011-04-348748
Descripción
Sumario:Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

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