Cargando…
A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax
Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in en...
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Hematology
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438884/ https://www.ncbi.nlm.nih.gov/pubmed/21768300 http://dx.doi.org/10.1182/blood-2011-04-348748 |
| _version_ | 1782242940727328768 |
|---|---|
| author | Russell, Bruce Suwanarusk, Rossarin Borlon, Céline Costa, Fabio T. M. Chu, Cindy S. Rijken, Marcus J. Sriprawat, Kanlaya Warter, Lucile Koh, Esther G. L. Malleret, Benoit Colin, Yves Bertrand, Olivier Adams, John H. D'Alessandro, Umberto Snounou, Georges Nosten, Francois Rénia, Laurent |
| author_facet | Russell, Bruce Suwanarusk, Rossarin Borlon, Céline Costa, Fabio T. M. Chu, Cindy S. Rijken, Marcus J. Sriprawat, Kanlaya Warter, Lucile Koh, Esther G. L. Malleret, Benoit Colin, Yves Bertrand, Olivier Adams, John H. D'Alessandro, Umberto Snounou, Georges Nosten, Francois Rénia, Laurent |
| author_sort | Russell, Bruce |
| collection | PubMed |
| description | Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand. |
| format | Online Article Text |
| id | pubmed-3438884 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2011 |
| publisher | American Society of Hematology |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-34388842012-09-11 A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax Russell, Bruce Suwanarusk, Rossarin Borlon, Céline Costa, Fabio T. M. Chu, Cindy S. Rijken, Marcus J. Sriprawat, Kanlaya Warter, Lucile Koh, Esther G. L. Malleret, Benoit Colin, Yves Bertrand, Olivier Adams, John H. D'Alessandro, Umberto Snounou, Georges Nosten, Francois Rénia, Laurent Blood Red Cells, Iron, and Erythropoiesis Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand. American Society of Hematology 2011-09-29 /pmc/articles/PMC3438884/ /pubmed/21768300 http://dx.doi.org/10.1182/blood-2011-04-348748 Text en © 2011 by The American Society of Hematology https://creativecommons.org/licenses/by-nc/3.0/us/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/ (https://creativecommons.org/licenses/by-nc/3.0/us/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Red Cells, Iron, and Erythropoiesis Russell, Bruce Suwanarusk, Rossarin Borlon, Céline Costa, Fabio T. M. Chu, Cindy S. Rijken, Marcus J. Sriprawat, Kanlaya Warter, Lucile Koh, Esther G. L. Malleret, Benoit Colin, Yves Bertrand, Olivier Adams, John H. D'Alessandro, Umberto Snounou, Georges Nosten, Francois Rénia, Laurent A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title | A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title_full | A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title_fullStr | A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title_full_unstemmed | A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title_short | A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax |
| title_sort | reliable ex vivo invasion assay of human reticulocytes by plasmodium vivax |
| topic | Red Cells, Iron, and Erythropoiesis |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438884/ https://www.ncbi.nlm.nih.gov/pubmed/21768300 http://dx.doi.org/10.1182/blood-2011-04-348748 |
| work_keys_str_mv | AT russellbruce areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT suwanaruskrossarin areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT borlonceline areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT costafabiotm areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT chucindys areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT rijkenmarcusj areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT sriprawatkanlaya areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT warterlucile areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT kohesthergl areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT malleretbenoit areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT colinyves areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT bertrandolivier areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT adamsjohnh areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT dalessandroumberto areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT snounougeorges areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT nostenfrancois areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT renialaurent areliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT russellbruce reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT suwanaruskrossarin reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT borlonceline reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT costafabiotm reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT chucindys reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT rijkenmarcusj reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT sriprawatkanlaya reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT warterlucile reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT kohesthergl reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT malleretbenoit reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT colinyves reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT bertrandolivier reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT adamsjohnh reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT dalessandroumberto reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT snounougeorges reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT nostenfrancois reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax AT renialaurent reliableexvivoinvasionassayofhumanreticulocytesbyplasmodiumvivax |