Cargando…

A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE

BACKGROUND: The green alga Chlamydomonas reinhardtii, although a premier model organism in biology, still lacks extensive insertion mutant libraries with well-identified Flanking Sequence Tags (FSTs). Rapid and efficient methods are needed for FST retrieval. RESULTS: Here, we present a novel method...

Descripción completa

Detalles Bibliográficos
Autores principales:	Meslet-Cladière, Laurence, Vallon, Olivier
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439250/ https://www.ncbi.nlm.nih.gov/pubmed/22735168 http://dx.doi.org/10.1186/1746-4811-8-21

Ejemplares similares

Halogenation in Fungi: What Do We Know and What Remains to Be Discovered?
por: Cochereau, Bastien, et al.
Publicado: (2022)

FSTVAL: a new web tool to validate bulk flanking sequence tags
por: Kim, Joung Sug, et al.
Publicado: (2012)

Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning
por: Wang, Zhen, et al.
Publicado: (2011)

Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii
por: Salinas-Giegé, Thalia, et al.
Publicado: (2017)

Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants
por: Gonzalez-Ballester, David, et al.
Publicado: (2011)

Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
por: Strenkert, Daniela, et al.
Publicado: (2011)

Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora
por: Genicot, Sabine M., et al.
Publicado: (2014)

TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation
por: Garcia-Echauri, Sergio A, et al.
Publicado: (2015)

Identification and Characterization of a New Type III Polyketide Synthase from a Marine Yeast, Naganishia uzbekistanensis
por: Martinelli, Laure, et al.
Publicado: (2020)

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds
por: Navarri, Marion, et al.
Publicado: (2016)

EST assembly supported by a draft genome sequence: an analysis of the Chlamydomonas reinhardtii transcriptome
por: Jain, Monica, et al.
Publicado: (2007)

Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
por: Cheng, Xi, et al.
Publicado: (2017)

Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags
por: Wu, Gary D, et al.
Publicado: (2010)

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii
por: Pollock, Steve V., et al.
Publicado: (2017)

Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism
por: Cavaiuolo, Marina, et al.
Publicado: (2017)

Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii
por: Cagnon, Caroline, et al.
Publicado: (2013)

New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas
por: Lin, Huawen, et al.
Publicado: (2013)

Comparison of a Label-Free Quantitative Proteomic Method Based on Peptide Ion Current Area to the Isotope Coded Affinity Tag Method
por: Ryu, Soyoung, et al.
Publicado: (2008)

Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions
por: Liang, Rubing, et al.
Publicado: (2010)

Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii
por: Toepel, Jörg, et al.
Publicado: (2011)

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
por: Scheich, Christoph, et al.
Publicado: (2003)

CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast
por: Berry, Lisa K., et al.
Publicado: (2020)

Architecture and evolution of subtelomeres in the unicellular green alga Chlamydomonas reinhardtii
por: Chaux-Jukic, Frédéric, et al.
Publicado: (2021)

Role of ClpP in the Biogenesis and Degradation of RuBisCO and ATP Synthase in Chlamydomonas reinhardtii
por: Majeran, Wojciech, et al.
Publicado: (2019)

Efficient chromatin profiling of H3K4me3 modification in cotton using CUT&Tag
por: Tao, Xiaoyuan, et al.
Publicado: (2020)

Sequence-based prediction of permissive stretches for internal protein tagging and knockdown
por: Oesterle, Sabine, et al.
Publicado: (2017)

Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes
por: Cao, Jiangling, et al.
Publicado: (2020)

Metabolic labeling with stable isotope nitrogen ((15)N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii
por: Sauer, Marie-Laure A, et al.
Publicado: (2014)

An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X protein
por: Rash, Adam, et al.
Publicado: (2014)

SeqURE – a new copy-capture based method for sequencing of unknown Retroposition events
por: Komkov, Alexander Y., et al.
Publicado: (2020)

Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii
por: Dutcher, Susan K., et al.
Publicado: (2012)

A novel method to identify gene interaction patterns
por: Lu, Xinguo, et al.
Publicado: (2021)

Nitrogen-dependent coordination of cell cycle, quiescence and TAG accumulation in Chlamydomonas
por: Takeuchi, Tomomi, et al.
Publicado: (2019)

Principles underlying the design of "The Number Race", an adaptive computer game for remediation of dyscalculia
por: Wilson, Anna J, et al.
Publicado: (2006)

Integration of Expressed Sequence Tag Data Flanking Predicted RNA Secondary Structures Facilitates Novel Non-Coding RNA Discovery
por: Krzyzanowski, Paul M., et al.
Publicado: (2011)

Approximation properties of haplotype tagging
por: Vinterbo, Staal A, et al.
Publicado: (2006)

High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging
por: Ehrenberg, Philip K, et al.
Publicado: (2014)

Dismantling "Race" in Health Research
por: Dordunoo, Dzifa, et al.
Publicado: (2022)

Identifying and measuring land-use and proximity conflicts: methods and identification
por: Torre, André, et al.
Publicado: (2014)

Median knock-down time as a new method for evaluating insecticide-treated textiles for mosquito control
por: Skovmand, Ole, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_o3ot9934lhtjhumk86v4f6dho4