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Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer
The molecular events that lead to malignant transformation and subsequent metastasis of breast carcinoma include alterations in the cells at genome, transcriptome and proteome levels. In this study, we used publicly available gene expression databases to identify those candidate genes which are upre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439379/ https://www.ncbi.nlm.nih.gov/pubmed/22709790 http://dx.doi.org/10.1186/1559-0275-9-7 |
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author | Telikicherla, Deepthi Marimuthu, Arivusudar Kashyap, Manoj Kumar Ramachandra, Y L Mohan, Sujatha Roa, Juan Carlos Maharudraiah, Jagadeesha Pandey, Akhilesh |
author_facet | Telikicherla, Deepthi Marimuthu, Arivusudar Kashyap, Manoj Kumar Ramachandra, Y L Mohan, Sujatha Roa, Juan Carlos Maharudraiah, Jagadeesha Pandey, Akhilesh |
author_sort | Telikicherla, Deepthi |
collection | PubMed |
description | The molecular events that lead to malignant transformation and subsequent metastasis of breast carcinoma include alterations in the cells at genome, transcriptome and proteome levels. In this study, we used publicly available gene expression databases to identify those candidate genes which are upregulated at the mRNA level in breast cancers but have not been systematically validated at the protein level. Based on an extensive literature search, we identified ribosome binding protein 1 (RRBP1) as a candidate that is upregulated at the mRNA level in five different studies but its protein expression had not been investigated. Immunohistochemical labeling of breast cancer tissue microarrays was carried out to determine the expression of RRBP1 in a large panel of breast cancers. We found that RRBP1 was overexpressed in 84% (177/219) of breast carcinoma cases tested. The subcellular localization of RRBP1 was mainly observed to be in the cytoplasm with intense staining in the perinuclear region. Our findings suggest that RRBP1 is an interesting molecule that can be further studied for its potential to serve as a breast cancer biomarker. This study also demonstrates how the integration of biological data from available resources in conjunction with systematic evaluation approaches can be successfully applied to clinical proteomics. |
format | Online Article Text |
id | pubmed-3439379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-34393792012-09-17 Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer Telikicherla, Deepthi Marimuthu, Arivusudar Kashyap, Manoj Kumar Ramachandra, Y L Mohan, Sujatha Roa, Juan Carlos Maharudraiah, Jagadeesha Pandey, Akhilesh Clin Proteomics Research The molecular events that lead to malignant transformation and subsequent metastasis of breast carcinoma include alterations in the cells at genome, transcriptome and proteome levels. In this study, we used publicly available gene expression databases to identify those candidate genes which are upregulated at the mRNA level in breast cancers but have not been systematically validated at the protein level. Based on an extensive literature search, we identified ribosome binding protein 1 (RRBP1) as a candidate that is upregulated at the mRNA level in five different studies but its protein expression had not been investigated. Immunohistochemical labeling of breast cancer tissue microarrays was carried out to determine the expression of RRBP1 in a large panel of breast cancers. We found that RRBP1 was overexpressed in 84% (177/219) of breast carcinoma cases tested. The subcellular localization of RRBP1 was mainly observed to be in the cytoplasm with intense staining in the perinuclear region. Our findings suggest that RRBP1 is an interesting molecule that can be further studied for its potential to serve as a breast cancer biomarker. This study also demonstrates how the integration of biological data from available resources in conjunction with systematic evaluation approaches can be successfully applied to clinical proteomics. Springer 2012-06-18 /pmc/articles/PMC3439379/ /pubmed/22709790 http://dx.doi.org/10.1186/1559-0275-9-7 Text en Copyright ©2012 Telikicherla et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Telikicherla, Deepthi Marimuthu, Arivusudar Kashyap, Manoj Kumar Ramachandra, Y L Mohan, Sujatha Roa, Juan Carlos Maharudraiah, Jagadeesha Pandey, Akhilesh Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title | Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title_full | Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title_fullStr | Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title_full_unstemmed | Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title_short | Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer |
title_sort | overexpression of ribosome binding protein 1 (rrbp1) in breast cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439379/ https://www.ncbi.nlm.nih.gov/pubmed/22709790 http://dx.doi.org/10.1186/1559-0275-9-7 |
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