Cargando…
Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate
BACKGROUND: Osteoarthritis (OA) is an important subtype of temporomandibular disorders. A simple and reproducible animal model that mimics the histopathologic changes, both in the cartilage and subchondral bone, and clinical symptoms of temporomandibular joint osteoarthritis (TMJOA) would help in ou...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439407/ https://www.ncbi.nlm.nih.gov/pubmed/22984604 http://dx.doi.org/10.1371/journal.pone.0045036 |
_version_ | 1782243000839045120 |
---|---|
author | Wang, Xue-Dong Kou, Xiao-Xing He, Dan-Qing Zeng, Min-Min Meng, Zhen Bi, Rui-Yun Liu, Yan Zhang, Jie-Ni Gan, Ye-Hua Zhou, Yan-Heng |
author_facet | Wang, Xue-Dong Kou, Xiao-Xing He, Dan-Qing Zeng, Min-Min Meng, Zhen Bi, Rui-Yun Liu, Yan Zhang, Jie-Ni Gan, Ye-Hua Zhou, Yan-Heng |
author_sort | Wang, Xue-Dong |
collection | PubMed |
description | BACKGROUND: Osteoarthritis (OA) is an important subtype of temporomandibular disorders. A simple and reproducible animal model that mimics the histopathologic changes, both in the cartilage and subchondral bone, and clinical symptoms of temporomandibular joint osteoarthritis (TMJOA) would help in our understanding of its process and underlying mechanism. OBJECTIVE: To explore whether injection of monosodium iodoacetate (MIA) into the upper compartment of rat TMJ could induce OA-like lesions. METHODS: Female rats were injected with varied doses of MIA into the upper compartment and observed for up to 12 weeks. Histologic, radiographic, behavioral, and molecular changes in the TMJ were evaluated by light and electron microscopy, MicroCT scanning, head withdrawal threshold test, real-time PCR, immunohistochemistry, and TUNEL assay. RESULTS: The intermediate zone of the disc loosened by 1 day post-MIA injection and thinned thereafter. Injection of an MIA dose of 0.5 mg or higher induced typical OA-like lesions in the TMJ within 4 weeks. Condylar destruction presented in a time-dependent manner, including chondrocyte apoptosis in the early stages, subsequent cartilage matrix disorganization and subchondral bone erosion, fibrosis, subchondral bone sclerosis, and osteophyte formation in the late stages. Nociceptive responses increased in the early stages, corresponding to severe synovitis. Furthermore, chondrocyte apoptosis and an imbalance between anabolism and catabolism of cartilage and subchondral bone might account for the condylar destruction. CONCLUSIONS: Multi-level data demonstrated a reliable and convenient rat model of TMJOA could be induced by MIA injection into the upper compartment. The model might facilitate TMJOA related researches. |
format | Online Article Text |
id | pubmed-3439407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34394072012-09-14 Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate Wang, Xue-Dong Kou, Xiao-Xing He, Dan-Qing Zeng, Min-Min Meng, Zhen Bi, Rui-Yun Liu, Yan Zhang, Jie-Ni Gan, Ye-Hua Zhou, Yan-Heng PLoS One Research Article BACKGROUND: Osteoarthritis (OA) is an important subtype of temporomandibular disorders. A simple and reproducible animal model that mimics the histopathologic changes, both in the cartilage and subchondral bone, and clinical symptoms of temporomandibular joint osteoarthritis (TMJOA) would help in our understanding of its process and underlying mechanism. OBJECTIVE: To explore whether injection of monosodium iodoacetate (MIA) into the upper compartment of rat TMJ could induce OA-like lesions. METHODS: Female rats were injected with varied doses of MIA into the upper compartment and observed for up to 12 weeks. Histologic, radiographic, behavioral, and molecular changes in the TMJ were evaluated by light and electron microscopy, MicroCT scanning, head withdrawal threshold test, real-time PCR, immunohistochemistry, and TUNEL assay. RESULTS: The intermediate zone of the disc loosened by 1 day post-MIA injection and thinned thereafter. Injection of an MIA dose of 0.5 mg or higher induced typical OA-like lesions in the TMJ within 4 weeks. Condylar destruction presented in a time-dependent manner, including chondrocyte apoptosis in the early stages, subsequent cartilage matrix disorganization and subchondral bone erosion, fibrosis, subchondral bone sclerosis, and osteophyte formation in the late stages. Nociceptive responses increased in the early stages, corresponding to severe synovitis. Furthermore, chondrocyte apoptosis and an imbalance between anabolism and catabolism of cartilage and subchondral bone might account for the condylar destruction. CONCLUSIONS: Multi-level data demonstrated a reliable and convenient rat model of TMJOA could be induced by MIA injection into the upper compartment. The model might facilitate TMJOA related researches. Public Library of Science 2012-09-11 /pmc/articles/PMC3439407/ /pubmed/22984604 http://dx.doi.org/10.1371/journal.pone.0045036 Text en © 2012 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Xue-Dong Kou, Xiao-Xing He, Dan-Qing Zeng, Min-Min Meng, Zhen Bi, Rui-Yun Liu, Yan Zhang, Jie-Ni Gan, Ye-Hua Zhou, Yan-Heng Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title | Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title_full | Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title_fullStr | Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title_full_unstemmed | Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title_short | Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate |
title_sort | progression of cartilage degradation, bone resorption and pain in rat temporomandibular joint osteoarthritis induced by injection of iodoacetate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439407/ https://www.ncbi.nlm.nih.gov/pubmed/22984604 http://dx.doi.org/10.1371/journal.pone.0045036 |
work_keys_str_mv | AT wangxuedong progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT kouxiaoxing progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT hedanqing progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT zengminmin progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT mengzhen progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT biruiyun progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT liuyan progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT zhangjieni progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT ganyehua progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate AT zhouyanheng progressionofcartilagedegradationboneresorptionandpaininrattemporomandibularjointosteoarthritisinducedbyinjectionofiodoacetate |