Bulged DNA substrates for identifying poxvirus resolvase inhibitors

Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replication, but clinically useful inhibitors have not been developed. Here, we report an assay for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput screening and mechanis...

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Detalles Bibliográficos
Autores principales: Culyba, Matthew, Hwang, Young, Attar, Sana, Madrid, Peter B., Bupp, James, Huryn, Donna, Sanchez, Luis, Grobler, Jay, Miller, Michael D., Bushman, Frederic D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439875/
https://www.ncbi.nlm.nih.gov/pubmed/22581770
http://dx.doi.org/10.1093/nar/gks325

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439875/
https://www.ncbi.nlm.nih.gov/pubmed/22581770
http://dx.doi.org/10.1093/nar/gks325

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