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Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes

Translational efficiency is controlled by tRNAs and other genome-encoded mechanisms. In organelles, translational processes are dramatically altered because of genome shrinkage and horizontal acquisition of gene products. The influence of genome reduction on translation in endosymbionts is largely u...

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Autores principales: Hansen, Allison K., Moran, Nancy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439896/
https://www.ncbi.nlm.nih.gov/pubmed/22689638
http://dx.doi.org/10.1093/nar/gks503
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author Hansen, Allison K.
Moran, Nancy A.
author_facet Hansen, Allison K.
Moran, Nancy A.
author_sort Hansen, Allison K.
collection PubMed
description Translational efficiency is controlled by tRNAs and other genome-encoded mechanisms. In organelles, translational processes are dramatically altered because of genome shrinkage and horizontal acquisition of gene products. The influence of genome reduction on translation in endosymbionts is largely unknown. Here, we investigate whether divergent lineages of Buchnera aphidicola, the reduced-genome bacterial endosymbiont of aphids, possess altered translational features compared with their free-living relative, Escherichia coli. Our RNAseq data support the hypothesis that translation is less optimal in Buchnera than in E. coli. We observed a specific, convergent, pattern of tRNA loss in Buchnera and other endosymbionts that have undergone genome shrinkage. Furthermore, many modified nucleoside pathways that are important for E. coli translation are lost in Buchnera. Additionally, Buchnera’s A + T compositional bias has resulted in reduced tRNA thermostability, and may have altered aminoacyl-tRNA synthetase recognition sites. Buchnera tRNA genes are shorter than those of E. coli, as the majority no longer has a genome-encoded 3' CCA; however, all the expressed, shortened tRNAs undergo 3′ CCA maturation. Moreover, expression of tRNA isoacceptors was not correlated with the usage of corresponding codons. Overall, our data suggest that endosymbiont genome evolution alters tRNA characteristics that are known to influence translational efficiency in their free-living relative.
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spelling pubmed-34398962012-09-12 Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes Hansen, Allison K. Moran, Nancy A. Nucleic Acids Res Genomics Translational efficiency is controlled by tRNAs and other genome-encoded mechanisms. In organelles, translational processes are dramatically altered because of genome shrinkage and horizontal acquisition of gene products. The influence of genome reduction on translation in endosymbionts is largely unknown. Here, we investigate whether divergent lineages of Buchnera aphidicola, the reduced-genome bacterial endosymbiont of aphids, possess altered translational features compared with their free-living relative, Escherichia coli. Our RNAseq data support the hypothesis that translation is less optimal in Buchnera than in E. coli. We observed a specific, convergent, pattern of tRNA loss in Buchnera and other endosymbionts that have undergone genome shrinkage. Furthermore, many modified nucleoside pathways that are important for E. coli translation are lost in Buchnera. Additionally, Buchnera’s A + T compositional bias has resulted in reduced tRNA thermostability, and may have altered aminoacyl-tRNA synthetase recognition sites. Buchnera tRNA genes are shorter than those of E. coli, as the majority no longer has a genome-encoded 3' CCA; however, all the expressed, shortened tRNAs undergo 3′ CCA maturation. Moreover, expression of tRNA isoacceptors was not correlated with the usage of corresponding codons. Overall, our data suggest that endosymbiont genome evolution alters tRNA characteristics that are known to influence translational efficiency in their free-living relative. Oxford University Press 2012-09 2012-06-09 /pmc/articles/PMC3439896/ /pubmed/22689638 http://dx.doi.org/10.1093/nar/gks503 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genomics
Hansen, Allison K.
Moran, Nancy A.
Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title_full Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title_fullStr Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title_full_unstemmed Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title_short Altered tRNA characteristics and 3′ maturation in bacterial symbionts with reduced genomes
title_sort altered trna characteristics and 3′ maturation in bacterial symbionts with reduced genomes
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439896/
https://www.ncbi.nlm.nih.gov/pubmed/22689638
http://dx.doi.org/10.1093/nar/gks503
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