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Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells

Streptococcus suis is an important swine pathogen and an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses towards S. suis, and strategies used by this pathogen for subversion of these responses is scarce. The objective of this study was to identify the immune...

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Autores principales: Lecours, Marie-Pier, Segura, Mariela, Fittipaldi, Nahuel, Rivest, Serge, Gottschalk, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440357/
https://www.ncbi.nlm.nih.gov/pubmed/22984550
http://dx.doi.org/10.1371/journal.pone.0044746
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author Lecours, Marie-Pier
Segura, Mariela
Fittipaldi, Nahuel
Rivest, Serge
Gottschalk, Marcelo
author_facet Lecours, Marie-Pier
Segura, Mariela
Fittipaldi, Nahuel
Rivest, Serge
Gottschalk, Marcelo
author_sort Lecours, Marie-Pier
collection PubMed
description Streptococcus suis is an important swine pathogen and an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses towards S. suis, and strategies used by this pathogen for subversion of these responses is scarce. The objective of this study was to identify the immune receptors involved in S. suis recognition by dendritic cells (DCs). Production of cytokines and expression of co-stimulatory molecules by DCs were shown to strongly rely on MyD88-dependent signaling pathways, suggesting that DCs recognize S. suis and become activated mostly through Toll-like receptor (TLR) signaling. Supporting this fact, TLR2(−/−) DCs were severely impaired in the release of several cytokines and the surface expression of CD86 and MHC-II. The release of IL-12p70 and CXC10, and the expression of CD40 were found to depend on signaling by both TLR2 and TLR9. The release of IL-23 and CXCL1 were partially dependent on NOD2. Finally, despite the fact that MyD88 signaling was crucial for DC activation and maturation, MyD88-dependent pathways were not implicated in S. suis internalization by DCs. This first study on receptors involved in DC activation by S. suis suggests a major involvement of MyD88 signaling pathways, mainly (but not exclusively) through TLR2. A multimodal recognition involving a combination of different receptors seems essential for DC effective response to S. suis.
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spelling pubmed-34403572012-09-14 Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells Lecours, Marie-Pier Segura, Mariela Fittipaldi, Nahuel Rivest, Serge Gottschalk, Marcelo PLoS One Research Article Streptococcus suis is an important swine pathogen and an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses towards S. suis, and strategies used by this pathogen for subversion of these responses is scarce. The objective of this study was to identify the immune receptors involved in S. suis recognition by dendritic cells (DCs). Production of cytokines and expression of co-stimulatory molecules by DCs were shown to strongly rely on MyD88-dependent signaling pathways, suggesting that DCs recognize S. suis and become activated mostly through Toll-like receptor (TLR) signaling. Supporting this fact, TLR2(−/−) DCs were severely impaired in the release of several cytokines and the surface expression of CD86 and MHC-II. The release of IL-12p70 and CXC10, and the expression of CD40 were found to depend on signaling by both TLR2 and TLR9. The release of IL-23 and CXCL1 were partially dependent on NOD2. Finally, despite the fact that MyD88 signaling was crucial for DC activation and maturation, MyD88-dependent pathways were not implicated in S. suis internalization by DCs. This first study on receptors involved in DC activation by S. suis suggests a major involvement of MyD88 signaling pathways, mainly (but not exclusively) through TLR2. A multimodal recognition involving a combination of different receptors seems essential for DC effective response to S. suis. Public Library of Science 2012-09-12 /pmc/articles/PMC3440357/ /pubmed/22984550 http://dx.doi.org/10.1371/journal.pone.0044746 Text en © 2012 Lecours et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lecours, Marie-Pier
Segura, Mariela
Fittipaldi, Nahuel
Rivest, Serge
Gottschalk, Marcelo
Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title_full Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title_fullStr Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title_full_unstemmed Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title_short Immune Receptors Involved in Streptococcus suis Recognition by Dendritic Cells
title_sort immune receptors involved in streptococcus suis recognition by dendritic cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440357/
https://www.ncbi.nlm.nih.gov/pubmed/22984550
http://dx.doi.org/10.1371/journal.pone.0044746
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