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Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure

The nucleoid of the planctomycete Gemmata obscuriglobus is unique within the Bacteria in being both highly condensed and enclosed by a double-membrane nuclear envelope, seemingly analogous to the nucleus of eukaryotes. Here we have applied electron tomography to study high-pressure frozen, cryosubst...

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Autores principales: Yee, Benjamin, Sagulenko, Evgeny, Morgan, Garry P., Webb, Richard I., Fuerst, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440768/
https://www.ncbi.nlm.nih.gov/pubmed/22993511
http://dx.doi.org/10.3389/fmicb.2012.00326
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author Yee, Benjamin
Sagulenko, Evgeny
Morgan, Garry P.
Webb, Richard I.
Fuerst, John A.
author_facet Yee, Benjamin
Sagulenko, Evgeny
Morgan, Garry P.
Webb, Richard I.
Fuerst, John A.
author_sort Yee, Benjamin
collection PubMed
description The nucleoid of the planctomycete Gemmata obscuriglobus is unique within the Bacteria in being both highly condensed and enclosed by a double-membrane nuclear envelope, seemingly analogous to the nucleus of eukaryotes. Here we have applied electron tomography to study high-pressure frozen, cryosubstituted cells of G. obscuriglobus and found multiple nested orders of DNA organization within the condensed nucleoid structure. Detailed examination of the nucleoid revealed a series of nested arcs characteristic of liquid crystalline cholesteric DNA structure. The finest fibers were arranged in parallel concentrically in a double-twist organization. At the highest order of nucleoid organization, several of these structures come together to form the core of the G. obscuriglobus nucleoid. The complex structure of DNA within this nucleoid may have implications for understanding the evolutionary significance of compartmentalized planctomycete cells.
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spelling pubmed-34407682012-09-19 Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure Yee, Benjamin Sagulenko, Evgeny Morgan, Garry P. Webb, Richard I. Fuerst, John A. Front Microbiol Microbiology The nucleoid of the planctomycete Gemmata obscuriglobus is unique within the Bacteria in being both highly condensed and enclosed by a double-membrane nuclear envelope, seemingly analogous to the nucleus of eukaryotes. Here we have applied electron tomography to study high-pressure frozen, cryosubstituted cells of G. obscuriglobus and found multiple nested orders of DNA organization within the condensed nucleoid structure. Detailed examination of the nucleoid revealed a series of nested arcs characteristic of liquid crystalline cholesteric DNA structure. The finest fibers were arranged in parallel concentrically in a double-twist organization. At the highest order of nucleoid organization, several of these structures come together to form the core of the G. obscuriglobus nucleoid. The complex structure of DNA within this nucleoid may have implications for understanding the evolutionary significance of compartmentalized planctomycete cells. Frontiers Media S.A. 2012-09-13 /pmc/articles/PMC3440768/ /pubmed/22993511 http://dx.doi.org/10.3389/fmicb.2012.00326 Text en Copyright © 2012 Yee, Sagulenko, Morgan, Webb and Fuerst. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Microbiology
Yee, Benjamin
Sagulenko, Evgeny
Morgan, Garry P.
Webb, Richard I.
Fuerst, John A.
Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title_full Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title_fullStr Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title_full_unstemmed Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title_short Electron tomography of the nucleoid of Gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
title_sort electron tomography of the nucleoid of gemmata obscuriglobus reveals complex liquid crystalline cholesteric structure
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440768/
https://www.ncbi.nlm.nih.gov/pubmed/22993511
http://dx.doi.org/10.3389/fmicb.2012.00326
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