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Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding

BACKGROUND: High molecular weight glutenin subunits (HMW-GSs), encoded by the genes at Glu-1 loci in wheat and its related species, are significant in the determination of grain processing quality. However, the diversity and variations of HMW-GSs are relatively low in bread wheat. More interests are...

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Autores principales: Jiang, Qian-Tao, Ma, Jian, Wei, Yu-Ming, Liu, Ya-Xi, Lan, Xiu-Jin, Dai, Shou-Fen, Lu, Zhen-Xiang, Zhao, Shan, Zhao, Quan-Zhi, Zheng, You-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441382/
https://www.ncbi.nlm.nih.gov/pubmed/22646663
http://dx.doi.org/10.1186/1471-2229-12-73
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author Jiang, Qian-Tao
Ma, Jian
Wei, Yu-Ming
Liu, Ya-Xi
Lan, Xiu-Jin
Dai, Shou-Fen
Lu, Zhen-Xiang
Zhao, Shan
Zhao, Quan-Zhi
Zheng, You-Liang
author_facet Jiang, Qian-Tao
Ma, Jian
Wei, Yu-Ming
Liu, Ya-Xi
Lan, Xiu-Jin
Dai, Shou-Fen
Lu, Zhen-Xiang
Zhao, Shan
Zhao, Quan-Zhi
Zheng, You-Liang
author_sort Jiang, Qian-Tao
collection PubMed
description BACKGROUND: High molecular weight glutenin subunits (HMW-GSs), encoded by the genes at Glu-1 loci in wheat and its related species, are significant in the determination of grain processing quality. However, the diversity and variations of HMW-GSs are relatively low in bread wheat. More interests are now focused on wheat wild relatives in Triticeae. The genus Aegilops represents an important germplasm for novel HWM-GSs and other useful genes for wheat genetic improvement. RESULTS: Six novel Glu-1 alleles and HMW-GSs were identified and characterized from three species of Aegilops section Sitopsis (S genome). Both open reading frames (ORFs) and promoter regions of these Glu-1 alleles were sequenced and characterized. The ORFs of Sitopsis Glu-1 genes are approximately 2.9 kb and 2.3 kb for x-type and y-type subunits, respectively. Although the primary structures of Sitopsis HMW-GSs are similar to those of previously reported ones, all six x-type or y-type subunits have the large fragment insertions. Our comparative analyses of the deduced amino acid sequences verified that Aegilops section Sitopsis species encode novel HMW-GSs with their molecular weights larger than almost all other known HMW-GSs. The Glu-1 promoter sequences share the high homology among S genome. Our phylogenetic analyses by both network and NJ tree indicated that there is a close phylogenetic evolutionary relationship of x-type and y-type subunit between S and D genome. CONCLUSIONS: The large molecular weight of HMW-GSs from S genome is a unique feature identified in this study. Such large subunits are resulted from the duplications of repetitive domains in Sitopsis HMW-GSs. The unequal crossover events are the most likely mechanism of variations in glutenin subunits. The S genome-encoded subunits, 1Dx2.2 and 1Dx2.2* have independent origins, although they share similar evolutionary mechanism. As HMW-GSs play a key role in wheat baking quality, these large Sitopsis glutenin subunits can be used as special genetic resources for wheat quality improvement.
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spelling pubmed-34413822012-09-14 Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding Jiang, Qian-Tao Ma, Jian Wei, Yu-Ming Liu, Ya-Xi Lan, Xiu-Jin Dai, Shou-Fen Lu, Zhen-Xiang Zhao, Shan Zhao, Quan-Zhi Zheng, You-Liang BMC Plant Biol Research Article BACKGROUND: High molecular weight glutenin subunits (HMW-GSs), encoded by the genes at Glu-1 loci in wheat and its related species, are significant in the determination of grain processing quality. However, the diversity and variations of HMW-GSs are relatively low in bread wheat. More interests are now focused on wheat wild relatives in Triticeae. The genus Aegilops represents an important germplasm for novel HWM-GSs and other useful genes for wheat genetic improvement. RESULTS: Six novel Glu-1 alleles and HMW-GSs were identified and characterized from three species of Aegilops section Sitopsis (S genome). Both open reading frames (ORFs) and promoter regions of these Glu-1 alleles were sequenced and characterized. The ORFs of Sitopsis Glu-1 genes are approximately 2.9 kb and 2.3 kb for x-type and y-type subunits, respectively. Although the primary structures of Sitopsis HMW-GSs are similar to those of previously reported ones, all six x-type or y-type subunits have the large fragment insertions. Our comparative analyses of the deduced amino acid sequences verified that Aegilops section Sitopsis species encode novel HMW-GSs with their molecular weights larger than almost all other known HMW-GSs. The Glu-1 promoter sequences share the high homology among S genome. Our phylogenetic analyses by both network and NJ tree indicated that there is a close phylogenetic evolutionary relationship of x-type and y-type subunit between S and D genome. CONCLUSIONS: The large molecular weight of HMW-GSs from S genome is a unique feature identified in this study. Such large subunits are resulted from the duplications of repetitive domains in Sitopsis HMW-GSs. The unequal crossover events are the most likely mechanism of variations in glutenin subunits. The S genome-encoded subunits, 1Dx2.2 and 1Dx2.2* have independent origins, although they share similar evolutionary mechanism. As HMW-GSs play a key role in wheat baking quality, these large Sitopsis glutenin subunits can be used as special genetic resources for wheat quality improvement. BioMed Central 2012-05-30 /pmc/articles/PMC3441382/ /pubmed/22646663 http://dx.doi.org/10.1186/1471-2229-12-73 Text en Copyright ©2012 Jiang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jiang, Qian-Tao
Ma, Jian
Wei, Yu-Ming
Liu, Ya-Xi
Lan, Xiu-Jin
Dai, Shou-Fen
Lu, Zhen-Xiang
Zhao, Shan
Zhao, Quan-Zhi
Zheng, You-Liang
Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title_full Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title_fullStr Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title_full_unstemmed Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title_short Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding
title_sort novel variants of hmw glutenin subunits from aegilops section sitopsis species in relation to evolution and wheat breeding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441382/
https://www.ncbi.nlm.nih.gov/pubmed/22646663
http://dx.doi.org/10.1186/1471-2229-12-73
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