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Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays

BACKGROUND: Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS: We optimized a Populus leaf mesophyll protoplast isolation protocol and establ...

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Detalles Bibliográficos
Autores principales:	Guo, Jianjun, Morrell-Falvey, Jennifer L., Labbé, Jessy L., Muchero, Wellington, Kalluri, Udaya C., Tuskan, Gerald A., Chen, Jin-Gui
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441479/ https://www.ncbi.nlm.nih.gov/pubmed/23028673 http://dx.doi.org/10.1371/journal.pone.0044908

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