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Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation

Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of the growth medium both in shaking flasks and bioreactor cultivation. The impact of medium...

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Autores principales: Brzonkalik, Katrin, Hümmer, Dominik, Syldatk, Christoph, Neumann, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441619/
https://www.ncbi.nlm.nih.gov/pubmed/22608165
http://dx.doi.org/10.1186/2191-0855-2-28
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author Brzonkalik, Katrin
Hümmer, Dominik
Syldatk, Christoph
Neumann, Anke
author_facet Brzonkalik, Katrin
Hümmer, Dominik
Syldatk, Christoph
Neumann, Anke
author_sort Brzonkalik, Katrin
collection PubMed
description Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of the growth medium both in shaking flasks and bioreactor cultivation. The impact of medium pH on mycotoxin production was studied in the range of pH 3.5 - 8. pH values above 5.5 led to a decreased mycotoxin production or inhibited mycotoxin formation completely whereas an acidic pH in the range of 4.0-4.5 was optimal for mycotoxin production. The influence of the C:N ratio was evaluated over the range of 24 to 96. Glucose was used as carbon source and its concentration was altered while nitrogen concentration was kept constant. Growth kinetics and mycotoxin production parameters were studied depending on different C:N ratios. With increasing initial glucose concentration fungal biomass did increase but the maximum specific growth rate was not influenced. The optimal initial C:N ratio for attaining highest mycotoxin concentrations was 72. A higher C:N ratio did not further enhance mycotoxin production.
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spelling pubmed-34416192012-09-17 Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation Brzonkalik, Katrin Hümmer, Dominik Syldatk, Christoph Neumann, Anke AMB Express Original Article Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of the growth medium both in shaking flasks and bioreactor cultivation. The impact of medium pH on mycotoxin production was studied in the range of pH 3.5 - 8. pH values above 5.5 led to a decreased mycotoxin production or inhibited mycotoxin formation completely whereas an acidic pH in the range of 4.0-4.5 was optimal for mycotoxin production. The influence of the C:N ratio was evaluated over the range of 24 to 96. Glucose was used as carbon source and its concentration was altered while nitrogen concentration was kept constant. Growth kinetics and mycotoxin production parameters were studied depending on different C:N ratios. With increasing initial glucose concentration fungal biomass did increase but the maximum specific growth rate was not influenced. The optimal initial C:N ratio for attaining highest mycotoxin concentrations was 72. A higher C:N ratio did not further enhance mycotoxin production. Springer 2012-05-20 /pmc/articles/PMC3441619/ /pubmed/22608165 http://dx.doi.org/10.1186/2191-0855-2-28 Text en Copyright ©2012 Brzonkalik et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Brzonkalik, Katrin
Hümmer, Dominik
Syldatk, Christoph
Neumann, Anke
Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title_full Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title_fullStr Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title_full_unstemmed Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title_short Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation
title_sort influence of ph and carbon to nitrogen ratio on mycotoxin production by alternaria alternata in submerged cultivation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441619/
https://www.ncbi.nlm.nih.gov/pubmed/22608165
http://dx.doi.org/10.1186/2191-0855-2-28
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