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Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility

The cytoplasmic dynein regulatory factor Lis1, which induces a persistent tight binding to microtubules and allows for transport of cargoes under high-load conditions, is also present in motile cilia/flagella. We observed that Lis1 levels in flagella of Chlamydomonas strains that exhibit defective m...

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Autores principales: Rompolas, Panteleimon, Patel-King, Ramila S., King, Stephen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442404/
https://www.ncbi.nlm.nih.gov/pubmed/22855525
http://dx.doi.org/10.1091/mbc.E12-04-0287
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author Rompolas, Panteleimon
Patel-King, Ramila S.
King, Stephen M.
author_facet Rompolas, Panteleimon
Patel-King, Ramila S.
King, Stephen M.
author_sort Rompolas, Panteleimon
collection PubMed
description The cytoplasmic dynein regulatory factor Lis1, which induces a persistent tight binding to microtubules and allows for transport of cargoes under high-load conditions, is also present in motile cilia/flagella. We observed that Lis1 levels in flagella of Chlamydomonas strains that exhibit defective motility due to mutation of various axonemal substructures were greatly enhanced compared with wild type; this increase was absolutely dependent on the presence within the flagellum of the outer arm dynein α heavy chain/light chain 5 thioredoxin unit. To assess whether cells might interpret defective motility as a “high-load environment,” we reduced the flagellar beat frequency of wild-type cells through enhanced viscous load and by reductive stress; both treatments resulted in increased levels of flagellar Lis1, which altered the intrinsic beat frequency of the trans flagellum. Differential extraction of Lis1 from wild-type and mutant axonemes suggests that the affinity of outer arm dynein for Lis1 is directly modulated. In cytoplasm, Lis1 localized to two punctate structures, one of which was located near the base of the flagella. These data reveal that the cell actively monitors motility and dynamically modulates flagellar levels of the dynein regulatory factor Lis1 in response to imposed alterations in beat parameters.
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spelling pubmed-34424042012-11-30 Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility Rompolas, Panteleimon Patel-King, Ramila S. King, Stephen M. Mol Biol Cell Articles The cytoplasmic dynein regulatory factor Lis1, which induces a persistent tight binding to microtubules and allows for transport of cargoes under high-load conditions, is also present in motile cilia/flagella. We observed that Lis1 levels in flagella of Chlamydomonas strains that exhibit defective motility due to mutation of various axonemal substructures were greatly enhanced compared with wild type; this increase was absolutely dependent on the presence within the flagellum of the outer arm dynein α heavy chain/light chain 5 thioredoxin unit. To assess whether cells might interpret defective motility as a “high-load environment,” we reduced the flagellar beat frequency of wild-type cells through enhanced viscous load and by reductive stress; both treatments resulted in increased levels of flagellar Lis1, which altered the intrinsic beat frequency of the trans flagellum. Differential extraction of Lis1 from wild-type and mutant axonemes suggests that the affinity of outer arm dynein for Lis1 is directly modulated. In cytoplasm, Lis1 localized to two punctate structures, one of which was located near the base of the flagella. These data reveal that the cell actively monitors motility and dynamically modulates flagellar levels of the dynein regulatory factor Lis1 in response to imposed alterations in beat parameters. The American Society for Cell Biology 2012-09-15 /pmc/articles/PMC3442404/ /pubmed/22855525 http://dx.doi.org/10.1091/mbc.E12-04-0287 Text en © 2012 Rompolas et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Rompolas, Panteleimon
Patel-King, Ramila S.
King, Stephen M.
Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title_full Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title_fullStr Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title_full_unstemmed Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title_short Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
title_sort association of lis1 with outer arm dynein is modulated in response to alterations in flagellar motility
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442404/
https://www.ncbi.nlm.nih.gov/pubmed/22855525
http://dx.doi.org/10.1091/mbc.E12-04-0287
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