Cargando…

Real-time quantitative PCR to discriminate and quantify lambdoid bacteriophages of Escherichia coli K-12

Quantification of bacteriophages by real-time quantitative PCR (qPCR) is an interesting alternative to the traditional plaque assay. Importantly, the method should in principle be able to discriminate between closely related phages that are indistinguishable by most other means. Here, a method is pr...

Descripción completa

Detalles Bibliográficos
Autor principal:	Refardt, Dominik
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Landes Bioscience 2012
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442831/ https://www.ncbi.nlm.nih.gov/pubmed/23050220 http://dx.doi.org/10.4161/bact.20092

Ejemplares similares

The Probiotic Escherichia coli Strain Nissle 1917 Combats Lambdoid Bacteriophages stx and λ
por: Bury, Susanne, et al.
Publicado: (2018)

Unauthorized Horizontal Spread in the Laboratory Environment: The Tactics of Lula, a Temperate Lambdoid Bacteriophage of Escherichia coli
por: Rotman, Ella, et al.
Publicado: (2010)

Defying bacteriophages: Contrasting altruistic with individual-based resistance mechanisms in Escherichia coli
por: Refardt, Dominik, et al.
Publicado: (2013)

Co-transfer of functionally interdependent genes contributes to genome mosaicism in lambdoid phages
por: Kupczok, Anne, et al.
Publicado: (2022)

Analysis of the lambdoid prophage element e14 in the E. coli K-12 genome
por: Mehta, Preeti, et al.
Publicado: (2004)

Replication Region Analysis Reveals Non-lambdoid Shiga Toxin Converting Bacteriophages
por: Llarena, Ann-Katrin, et al.
Publicado: (2021)

Inhibition of spontaneous induction of lambdoid prophages in Escherichia coli cultures: simple procedures with possible biotechnological applications
por: Czyz, Agata, et al.
Publicado: (2001)

Pseudomonas Phage MD8: Genetic Mosaicism and Challenges of Taxonomic Classification of Lambdoid Bacteriophages
por: Evseev, Peter, et al.
Publicado: (2021)

Lambdoid synostosis: endoscopic craniectomy
por: Jimenez, David F.
Publicado: (2021)

Measurement and Diagnosis of Lambdoid Craniosynostosis
por: Blondin, Mario, et al.
Publicado: (2023)

Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways
por: Bookout, Angie L., et al.
Publicado: (2003)

Enhancing protein perdeuteration by experimental evolution of Escherichia coli K‐12 for rapid growth in deuterium‐based media
por: Kelpšas, Vinardas, et al.
Publicado: (2021)

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR
por: Zhou, Kang, et al.
Publicado: (2011)

Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12
por: Poteete, Anthony R, et al.
Publicado: (2004)

Detecting, quantifying, and discriminating the mechanism of mosaic chromosomal aneuploidies using MAD-seq
por: Kong, Yu, et al.
Publicado: (2018)

Efficiency of induction of Shiga-toxin lambdoid prophages in Escherichia coli due to oxidative and antibiotic stress depends on the combination of prophage and the bacterial strain
por: Filipiak, Michalina, et al.
Publicado: (2019)

qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
por: Hellemans, Jan, et al.
Publicado: (2007)

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
por: Marullo, Manuela, et al.
Publicado: (2010)

PROPERTIES OF A BACTERIOPHAGE DERIVED FROM ESCHERICHIA COLI K235
por: Jesaitis, Margeris A., et al.
Publicado: (1963)

A new method for robust quantitative and qualitative analysis of real-time PCR
por: Shain, Eric B., et al.
Publicado: (2008)

Genomic DNA functions as a universal external standard in quantitative real-time PCR
por: Yun, James J., et al.
Publicado: (2006)

A one-step method for quantitative determination of uracil in DNA by real-time PCR
por: Horváth, András, et al.
Publicado: (2010)

Absolute quantitation of poly(R)-3-hydroxybutyric acid using spectrofluorometry in recombinant Escherichia coli
por: Rajankar, Mayooreshwar P, et al.
Publicado: (2018)

Nanopore sequencing of RNA and cDNA molecules in Escherichia coli
por: Grünberger, Felix, et al.
Publicado: (2022)

Frontofacial Features of Unilateral Lambdoid Craniosynostosis: A Multicenter Assessment
por: Lee, Jonathan, et al.
Publicado: (2023)

Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
por: Hilscher, Chelsey, et al.
Publicado: (2005)

Rapid and Accurate Detection of Bacteriophage Activity against Escherichia coli O157:H7 by Propidium Monoazide Real-Time PCR
por: Liu, Hui, et al.
Publicado: (2014)

Systematic identification and quantification of phase variation in commensal and pathogenic Escherichia coli
por: Goldberg, Amir, et al.
Publicado: (2014)

The Essential Genome of Escherichia coli K-12
por: Goodall, Emily C. A., et al.
Publicado: (2018)

External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
por: Vermeulen, Joëlle, et al.
Publicado: (2009)

Protection of bacteriophage-sensitive Escherichia coli by lysogens
por: Brown, Stanley, et al.
Publicado: (2022)

An optimized protocol for stepwise optimization of real-time RT-PCR analysis
por: Zhao, Fangzhou, et al.
Publicado: (2021)

Calvarial defects in the region of the lambdoid suture in neurofibromatosis type-1 patients
por: Solanki, Chirag, et al.
Publicado: (2015)

Computational modelling of patient specific spring assisted lambdoid craniosynostosis correction
por: Bozkurt, Selim, et al.
Publicado: (2020)

Nanoliter high throughput quantitative PCR
por: Morrison, Tom, et al.
Publicado: (2006)

Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro
por: Rahimzadeh, Golnar, et al.
Publicado: (2020)

Differential Codon Adaptation between dsDNA and ssDNA Phages in Escherichia coli
por: Chithambaram, Shivapriya, et al.
Publicado: (2014)

Multidimensional annotation of the Escherichia coli K-12 genome
por: Karp, Peter D., et al.
Publicado: (2007)

Characterization of Pyruvate Uptake in Escherichia coli K-12
por: Kreth, Jens, et al.
Publicado: (2013)

Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells
por: Møller-Olsen, Christian, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_67urp3v8ogrvsj9mfkt4s5h818