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Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures

BACKGROUND: The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify I...

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Detalles Bibliográficos
Autores principales:	Fernández-Castané, Alfred, Caminal, Glòria, López-Santín, Josep
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442970/ https://www.ncbi.nlm.nih.gov/pubmed/22571410 http://dx.doi.org/10.1186/1475-2859-11-58

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