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Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations
BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. The underlying mechanisms are unclear. RESULTS: EPCs from CB expressed abundant genes involved in cell cycle, hypoxi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443421/ https://www.ncbi.nlm.nih.gov/pubmed/22943456 http://dx.doi.org/10.1186/1471-2164-13-447 |
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author | Cheng, Cheng-Chung Lo, Hung-Hao Huang, Tse-Shun Cheng, Yi-Chieh Chang, Shi-Ting Chang, Shing-Jyh Wang, Hsei-Wei |
author_facet | Cheng, Cheng-Chung Lo, Hung-Hao Huang, Tse-Shun Cheng, Yi-Chieh Chang, Shi-Ting Chang, Shing-Jyh Wang, Hsei-Wei |
author_sort | Cheng, Cheng-Chung |
collection | PubMed |
description | BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. The underlying mechanisms are unclear. RESULTS: EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. smRNA-seq further deciphered miRNome patterns in EPCs isolated from CB or PB: 54 miRNAs were enriched in CB-EPCs, while another 50 in PB-EPCs. Specifically, CB-EPCs expressed more angiogenic miRNAs such as miR-31, while PB-EPCs possessed more tumor suppressive miRNAs including miR-10a. Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. CONCLUSIONS: Our results show the foundation for a more detailed understanding of EPCs from different anatomic sources. Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. |
format | Online Article Text |
id | pubmed-3443421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34434212012-09-16 Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations Cheng, Cheng-Chung Lo, Hung-Hao Huang, Tse-Shun Cheng, Yi-Chieh Chang, Shi-Ting Chang, Shing-Jyh Wang, Hsei-Wei BMC Genomics Research Article BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. The underlying mechanisms are unclear. RESULTS: EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. smRNA-seq further deciphered miRNome patterns in EPCs isolated from CB or PB: 54 miRNAs were enriched in CB-EPCs, while another 50 in PB-EPCs. Specifically, CB-EPCs expressed more angiogenic miRNAs such as miR-31, while PB-EPCs possessed more tumor suppressive miRNAs including miR-10a. Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. CONCLUSIONS: Our results show the foundation for a more detailed understanding of EPCs from different anatomic sources. Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. BioMed Central 2012-09-03 /pmc/articles/PMC3443421/ /pubmed/22943456 http://dx.doi.org/10.1186/1471-2164-13-447 Text en Copyright ©2012 Cheng et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cheng, Cheng-Chung Lo, Hung-Hao Huang, Tse-Shun Cheng, Yi-Chieh Chang, Shi-Ting Chang, Shing-Jyh Wang, Hsei-Wei Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title | Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title_full | Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title_fullStr | Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title_full_unstemmed | Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title_short | Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
title_sort | genetic module and mirnome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443421/ https://www.ncbi.nlm.nih.gov/pubmed/22943456 http://dx.doi.org/10.1186/1471-2164-13-447 |
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