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ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data

BACKGROUND: Measuring gene transcription using real-time reverse transcription polymerase chain reaction (RT-qPCR) technology is a mainstay of molecular biology. Technologies now exist to measure the abundance of many transcripts in parallel. The selection of the optimal reference gene for the norma...

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Detalles Bibliográficos
Autores principales:	Perkins, James R, Dawes, John M, McMahon, Steve B, Bennett, David LH, Orengo, Christine, Kohl, Matthias
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Software
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443438/ https://www.ncbi.nlm.nih.gov/pubmed/22748112 http://dx.doi.org/10.1186/1471-2164-13-296

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