A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins

Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryot...

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Autores principales: Lengsfeld, Bettina M., Berry, Kayla N., Ghosh, Sharmistha, Takahashi, Masateru, Francis, Nicole J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443814/
https://www.ncbi.nlm.nih.gov/pubmed/22993687
http://dx.doi.org/10.1038/srep00661
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author Lengsfeld, Bettina M.
Berry, Kayla N.
Ghosh, Sharmistha
Takahashi, Masateru
Francis, Nicole J.
author_facet Lengsfeld, Bettina M.
Berry, Kayla N.
Ghosh, Sharmistha
Takahashi, Masateru
Francis, Nicole J.
author_sort Lengsfeld, Bettina M.
collection PubMed
description Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.
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spelling pubmed-34438142012-09-19 A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins Lengsfeld, Bettina M. Berry, Kayla N. Ghosh, Sharmistha Takahashi, Masateru Francis, Nicole J. Sci Rep Article Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork. Nature Publishing Group 2012-09-17 /pmc/articles/PMC3443814/ /pubmed/22993687 http://dx.doi.org/10.1038/srep00661 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Lengsfeld, Bettina M.
Berry, Kayla N.
Ghosh, Sharmistha
Takahashi, Masateru
Francis, Nicole J.
A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title_full A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title_fullStr A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title_full_unstemmed A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title_short A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
title_sort polycomb complex remains bound through dna replication in the absence of other eukaryotic proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443814/
https://www.ncbi.nlm.nih.gov/pubmed/22993687
http://dx.doi.org/10.1038/srep00661
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