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TULIPs: Tunable, light-controlled interacting protein tags for cell biology

Naturally photoswitchable proteins offer a means of directly manipulating the formation of protein complexes that drive a diversity of cellular processes. We have developed tunable light-inducible dimerization tags (TULIPs) based on a synthetic interaction between the LOV2 domain of Avena sativa pho...

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Autores principales: Strickland, Devin, Lin, Yuan, Wagner, Elizabeth, Hope, C. Matthew, Zayner, Josiah, Antoniou, Chloe, Sosnick, Tobin R., Weiss, Eric L., Glotzer, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444151/
https://www.ncbi.nlm.nih.gov/pubmed/22388287
http://dx.doi.org/10.1038/nmeth.1904
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author Strickland, Devin
Lin, Yuan
Wagner, Elizabeth
Hope, C. Matthew
Zayner, Josiah
Antoniou, Chloe
Sosnick, Tobin R.
Weiss, Eric L.
Glotzer, Michael
author_facet Strickland, Devin
Lin, Yuan
Wagner, Elizabeth
Hope, C. Matthew
Zayner, Josiah
Antoniou, Chloe
Sosnick, Tobin R.
Weiss, Eric L.
Glotzer, Michael
author_sort Strickland, Devin
collection PubMed
description Naturally photoswitchable proteins offer a means of directly manipulating the formation of protein complexes that drive a diversity of cellular processes. We have developed tunable light-inducible dimerization tags (TULIPs) based on a synthetic interaction between the LOV2 domain of Avena sativa phototropin 1 (AsLOV2) and an engineered PDZ domain (ePDZ). TULIP tags can recruit proteins to diverse structures in living yeast and mammalian cells, either globally or with precise spatial control using a steerable laser. The equilibrium binding and kinetic parameters of the interaction are tunable by mutation, making TULIPs readily adaptable to signaling pathways with varying sensitivities and response times. We demonstrate the utility of TULIPs by conferring light sensitivity to functionally distinct components of the yeast mating pathway and by directing the site of cell polarization.
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spelling pubmed-34441512012-10-01 TULIPs: Tunable, light-controlled interacting protein tags for cell biology Strickland, Devin Lin, Yuan Wagner, Elizabeth Hope, C. Matthew Zayner, Josiah Antoniou, Chloe Sosnick, Tobin R. Weiss, Eric L. Glotzer, Michael Nat Methods Article Naturally photoswitchable proteins offer a means of directly manipulating the formation of protein complexes that drive a diversity of cellular processes. We have developed tunable light-inducible dimerization tags (TULIPs) based on a synthetic interaction between the LOV2 domain of Avena sativa phototropin 1 (AsLOV2) and an engineered PDZ domain (ePDZ). TULIP tags can recruit proteins to diverse structures in living yeast and mammalian cells, either globally or with precise spatial control using a steerable laser. The equilibrium binding and kinetic parameters of the interaction are tunable by mutation, making TULIPs readily adaptable to signaling pathways with varying sensitivities and response times. We demonstrate the utility of TULIPs by conferring light sensitivity to functionally distinct components of the yeast mating pathway and by directing the site of cell polarization. 2012-03-04 /pmc/articles/PMC3444151/ /pubmed/22388287 http://dx.doi.org/10.1038/nmeth.1904 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Strickland, Devin
Lin, Yuan
Wagner, Elizabeth
Hope, C. Matthew
Zayner, Josiah
Antoniou, Chloe
Sosnick, Tobin R.
Weiss, Eric L.
Glotzer, Michael
TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title_full TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title_fullStr TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title_full_unstemmed TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title_short TULIPs: Tunable, light-controlled interacting protein tags for cell biology
title_sort tulips: tunable, light-controlled interacting protein tags for cell biology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444151/
https://www.ncbi.nlm.nih.gov/pubmed/22388287
http://dx.doi.org/10.1038/nmeth.1904
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