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Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus

The present study was carried out to investigate the role of reactive oxygen species (ROS) metabolism in symptom development and pathogenesis in Nicotiana benthamiana plants upon infection with two strains of Pepper mild mottle virus, the Italian (PMMoV-I) and the Spanish (PMMoV-S) strains. In this...

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Autores principales: Hakmaoui, A., Pérez-Bueno, M. L., García-Fontana, B., Camejo, D., Jiménez, A., Sevilla, F., Barón, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444274/
https://www.ncbi.nlm.nih.gov/pubmed/22915745
http://dx.doi.org/10.1093/jxb/ers212
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author Hakmaoui, A.
Pérez-Bueno, M. L.
García-Fontana, B.
Camejo, D.
Jiménez, A.
Sevilla, F.
Barón, M.
author_facet Hakmaoui, A.
Pérez-Bueno, M. L.
García-Fontana, B.
Camejo, D.
Jiménez, A.
Sevilla, F.
Barón, M.
author_sort Hakmaoui, A.
collection PubMed
description The present study was carried out to investigate the role of reactive oxygen species (ROS) metabolism in symptom development and pathogenesis in Nicotiana benthamiana plants upon infection with two strains of Pepper mild mottle virus, the Italian (PMMoV-I) and the Spanish (PMMoV-S) strains. In this host, it has been shown that PMMoV-I is less virulent and plants show the capability to recover 21 d after inoculation. Analyses of oxidative stress biomarkers, ROS-scavenging enzyme activities, and antioxidant compounds were conducted in plants at different post-infection times. Only PMMoV-I stimulated a defence response through: (i) up-regulation of different superoxide dismutase isozymes; (ii) maintenance of adequate levels of three peroxiredoxins (2-Cys Prx, Prx IIC, and Prx IIF); and (iii) adjustments in the glutathione pool to maintain the total glutathione content. Moreover, there was an increase in the level of oxidized glutathione and ascorbate in the recovery phase of PMMoV-I-infected plants. The antioxidant response and the extent of oxidative stress in N. benthamiana plants correlates to: (i) the severity of the symptoms elicited by either strain of PMMoV; and (ii) the high capacity of PMMoV-I-infected plants for symptom recovery and delayed senescence, compared with PMMoV-S-infected plants.
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spelling pubmed-34442742012-09-19 Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus Hakmaoui, A. Pérez-Bueno, M. L. García-Fontana, B. Camejo, D. Jiménez, A. Sevilla, F. Barón, M. J Exp Bot Research Paper The present study was carried out to investigate the role of reactive oxygen species (ROS) metabolism in symptom development and pathogenesis in Nicotiana benthamiana plants upon infection with two strains of Pepper mild mottle virus, the Italian (PMMoV-I) and the Spanish (PMMoV-S) strains. In this host, it has been shown that PMMoV-I is less virulent and plants show the capability to recover 21 d after inoculation. Analyses of oxidative stress biomarkers, ROS-scavenging enzyme activities, and antioxidant compounds were conducted in plants at different post-infection times. Only PMMoV-I stimulated a defence response through: (i) up-regulation of different superoxide dismutase isozymes; (ii) maintenance of adequate levels of three peroxiredoxins (2-Cys Prx, Prx IIC, and Prx IIF); and (iii) adjustments in the glutathione pool to maintain the total glutathione content. Moreover, there was an increase in the level of oxidized glutathione and ascorbate in the recovery phase of PMMoV-I-infected plants. The antioxidant response and the extent of oxidative stress in N. benthamiana plants correlates to: (i) the severity of the symptoms elicited by either strain of PMMoV; and (ii) the high capacity of PMMoV-I-infected plants for symptom recovery and delayed senescence, compared with PMMoV-S-infected plants. Oxford University Press 2012-09 2012-08-21 /pmc/articles/PMC3444274/ /pubmed/22915745 http://dx.doi.org/10.1093/jxb/ers212 Text en © 2012 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Hakmaoui, A.
Pérez-Bueno, M. L.
García-Fontana, B.
Camejo, D.
Jiménez, A.
Sevilla, F.
Barón, M.
Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title_full Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title_fullStr Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title_full_unstemmed Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title_short Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus
title_sort analysis of the antioxidant response of nicotiana benthamiana to infection with two strains of pepper mild mottle virus
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444274/
https://www.ncbi.nlm.nih.gov/pubmed/22915745
http://dx.doi.org/10.1093/jxb/ers212
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