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SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444279/ https://www.ncbi.nlm.nih.gov/pubmed/22915741 http://dx.doi.org/10.1093/jxb/ers220 |
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author | Loukehaich, Rachid Wang, Taotao Ouyang, Bo Ziaf, Khurram Li, Hanxia Zhang, Junhong Lu, Yongen Ye, Zhibiao |
author_facet | Loukehaich, Rachid Wang, Taotao Ouyang, Bo Ziaf, Khurram Li, Hanxia Zhang, Junhong Lu, Yongen Ye, Zhibiao |
author_sort | Loukehaich, Rachid |
collection | PubMed |
description | Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP transcript is abundantly accumulated in leaf stomata and its expression varied with the circadian rhythm. SpUSP was remarkably induced by dehydration, salt stress, oxidative stress, and the phytohormone abscisic acid (ABA) etc. This protein was predominantly localized in the nucleus and cell membrane. Overexpressing SpUSP increased drought tolerance of tomato in the seedling and adult stages. Under drought stress, the ABA content significantly increased in the SpUSP-overexpressing plants, which induced stomatal closure and reduced water loss, leading to the enhancement of drought tolerance. Based on the microarray data, a large number of chlorophyll a/b-binding proteins and photosystem-related genes were up-regulated in the SpUSP-overexpressing plants under drought conditions, which possibly enhanced the stomatal sensivitity to ABA and maintained the photosynthetic function. SpUSP overexpression also alleviated the oxidative damage accompanied by oxidative stress-responsive gene activation and osmolyte accumulation. Annexin (SGN-U314161) was found to interacte with SpUSP in the yeast two-hybrid method. This interaction was further confirmed by the bimolecular fluorescence complementation assay. The present study demonstrated that the annexin-interacting SpUSP plays important roles in the drought tolerance of tomato by influencing ABA-induced stomatal movement, increasing photosynthesis, and alleviating oxidative stress. |
format | Online Article Text |
id | pubmed-3444279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34442792012-09-19 SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato Loukehaich, Rachid Wang, Taotao Ouyang, Bo Ziaf, Khurram Li, Hanxia Zhang, Junhong Lu, Yongen Ye, Zhibiao J Exp Bot Research Paper Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP transcript is abundantly accumulated in leaf stomata and its expression varied with the circadian rhythm. SpUSP was remarkably induced by dehydration, salt stress, oxidative stress, and the phytohormone abscisic acid (ABA) etc. This protein was predominantly localized in the nucleus and cell membrane. Overexpressing SpUSP increased drought tolerance of tomato in the seedling and adult stages. Under drought stress, the ABA content significantly increased in the SpUSP-overexpressing plants, which induced stomatal closure and reduced water loss, leading to the enhancement of drought tolerance. Based on the microarray data, a large number of chlorophyll a/b-binding proteins and photosystem-related genes were up-regulated in the SpUSP-overexpressing plants under drought conditions, which possibly enhanced the stomatal sensivitity to ABA and maintained the photosynthetic function. SpUSP overexpression also alleviated the oxidative damage accompanied by oxidative stress-responsive gene activation and osmolyte accumulation. Annexin (SGN-U314161) was found to interacte with SpUSP in the yeast two-hybrid method. This interaction was further confirmed by the bimolecular fluorescence complementation assay. The present study demonstrated that the annexin-interacting SpUSP plays important roles in the drought tolerance of tomato by influencing ABA-induced stomatal movement, increasing photosynthesis, and alleviating oxidative stress. Oxford University Press 2012-09 2012-08-21 /pmc/articles/PMC3444279/ /pubmed/22915741 http://dx.doi.org/10.1093/jxb/ers220 Text en © 2012 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Loukehaich, Rachid Wang, Taotao Ouyang, Bo Ziaf, Khurram Li, Hanxia Zhang, Junhong Lu, Yongen Ye, Zhibiao SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title | SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title_full | SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title_fullStr | SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title_full_unstemmed | SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title_short | SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
title_sort | spusp, an annexin-interacting universal stress protein, enhances drought tolerance in tomato |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444279/ https://www.ncbi.nlm.nih.gov/pubmed/22915741 http://dx.doi.org/10.1093/jxb/ers220 |
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