Cargando…

SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP t...

Descripción completa

Detalles Bibliográficos
Autores principales: Loukehaich, Rachid, Wang, Taotao, Ouyang, Bo, Ziaf, Khurram, Li, Hanxia, Zhang, Junhong, Lu, Yongen, Ye, Zhibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444279/
https://www.ncbi.nlm.nih.gov/pubmed/22915741
http://dx.doi.org/10.1093/jxb/ers220
_version_ 1782243653229477888
author Loukehaich, Rachid
Wang, Taotao
Ouyang, Bo
Ziaf, Khurram
Li, Hanxia
Zhang, Junhong
Lu, Yongen
Ye, Zhibiao
author_facet Loukehaich, Rachid
Wang, Taotao
Ouyang, Bo
Ziaf, Khurram
Li, Hanxia
Zhang, Junhong
Lu, Yongen
Ye, Zhibiao
author_sort Loukehaich, Rachid
collection PubMed
description Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP transcript is abundantly accumulated in leaf stomata and its expression varied with the circadian rhythm. SpUSP was remarkably induced by dehydration, salt stress, oxidative stress, and the phytohormone abscisic acid (ABA) etc. This protein was predominantly localized in the nucleus and cell membrane. Overexpressing SpUSP increased drought tolerance of tomato in the seedling and adult stages. Under drought stress, the ABA content significantly increased in the SpUSP-overexpressing plants, which induced stomatal closure and reduced water loss, leading to the enhancement of drought tolerance. Based on the microarray data, a large number of chlorophyll a/b-binding proteins and photosystem-related genes were up-regulated in the SpUSP-overexpressing plants under drought conditions, which possibly enhanced the stomatal sensivitity to ABA and maintained the photosynthetic function. SpUSP overexpression also alleviated the oxidative damage accompanied by oxidative stress-responsive gene activation and osmolyte accumulation. Annexin (SGN-U314161) was found to interacte with SpUSP in the yeast two-hybrid method. This interaction was further confirmed by the bimolecular fluorescence complementation assay. The present study demonstrated that the annexin-interacting SpUSP plays important roles in the drought tolerance of tomato by influencing ABA-induced stomatal movement, increasing photosynthesis, and alleviating oxidative stress.
format Online
Article
Text
id pubmed-3444279
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-34442792012-09-19 SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato Loukehaich, Rachid Wang, Taotao Ouyang, Bo Ziaf, Khurram Li, Hanxia Zhang, Junhong Lu, Yongen Ye, Zhibiao J Exp Bot Research Paper Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP transcript is abundantly accumulated in leaf stomata and its expression varied with the circadian rhythm. SpUSP was remarkably induced by dehydration, salt stress, oxidative stress, and the phytohormone abscisic acid (ABA) etc. This protein was predominantly localized in the nucleus and cell membrane. Overexpressing SpUSP increased drought tolerance of tomato in the seedling and adult stages. Under drought stress, the ABA content significantly increased in the SpUSP-overexpressing plants, which induced stomatal closure and reduced water loss, leading to the enhancement of drought tolerance. Based on the microarray data, a large number of chlorophyll a/b-binding proteins and photosystem-related genes were up-regulated in the SpUSP-overexpressing plants under drought conditions, which possibly enhanced the stomatal sensivitity to ABA and maintained the photosynthetic function. SpUSP overexpression also alleviated the oxidative damage accompanied by oxidative stress-responsive gene activation and osmolyte accumulation. Annexin (SGN-U314161) was found to interacte with SpUSP in the yeast two-hybrid method. This interaction was further confirmed by the bimolecular fluorescence complementation assay. The present study demonstrated that the annexin-interacting SpUSP plays important roles in the drought tolerance of tomato by influencing ABA-induced stomatal movement, increasing photosynthesis, and alleviating oxidative stress. Oxford University Press 2012-09 2012-08-21 /pmc/articles/PMC3444279/ /pubmed/22915741 http://dx.doi.org/10.1093/jxb/ers220 Text en © 2012 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Loukehaich, Rachid
Wang, Taotao
Ouyang, Bo
Ziaf, Khurram
Li, Hanxia
Zhang, Junhong
Lu, Yongen
Ye, Zhibiao
SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title_full SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title_fullStr SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title_full_unstemmed SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title_short SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
title_sort spusp, an annexin-interacting universal stress protein, enhances drought tolerance in tomato
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444279/
https://www.ncbi.nlm.nih.gov/pubmed/22915741
http://dx.doi.org/10.1093/jxb/ers220
work_keys_str_mv AT loukehaichrachid spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT wangtaotao spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT ouyangbo spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT ziafkhurram spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT lihanxia spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT zhangjunhong spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT luyongen spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato
AT yezhibiao spuspanannexininteractinguniversalstressproteinenhancesdroughttoleranceintomato