Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources

We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than con...

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Detalles Bibliográficos
Autores principales: Morisaka, Hironobu, Matsui, Kazuma, Tatsukami, Yohei, Kuroda, Kouichi, Miyake, Hideo, Tamaru, Yutaka, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444338/
https://www.ncbi.nlm.nih.gov/pubmed/22839966
http://dx.doi.org/10.1186/2191-0855-2-37
Descripción
Sumario:We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.

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