Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources

We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than con...

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Detalles Bibliográficos
Autores principales: Morisaka, Hironobu, Matsui, Kazuma, Tatsukami, Yohei, Kuroda, Kouichi, Miyake, Hideo, Tamaru, Yutaka, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444338/
https://www.ncbi.nlm.nih.gov/pubmed/22839966
http://dx.doi.org/10.1186/2191-0855-2-37
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author Morisaka, Hironobu
Matsui, Kazuma
Tatsukami, Yohei
Kuroda, Kouichi
Miyake, Hideo
Tamaru, Yutaka
Ueda, Mitsuyoshi
author_facet Morisaka, Hironobu
Matsui, Kazuma
Tatsukami, Yohei
Kuroda, Kouichi
Miyake, Hideo
Tamaru, Yutaka
Ueda, Mitsuyoshi
author_sort Morisaka, Hironobu
collection PubMed
description We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.
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spelling pubmed-34443382012-09-18 Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources Morisaka, Hironobu Matsui, Kazuma Tatsukami, Yohei Kuroda, Kouichi Miyake, Hideo Tamaru, Yutaka Ueda, Mitsuyoshi AMB Express Original Article We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components. Springer 2012-07-29 /pmc/articles/PMC3444338/ /pubmed/22839966 http://dx.doi.org/10.1186/2191-0855-2-37 Text en Copyright ©2012 Morisaka et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Morisaka, Hironobu
Matsui, Kazuma
Tatsukami, Yohei
Kuroda, Kouichi
Miyake, Hideo
Tamaru, Yutaka
Ueda, Mitsuyoshi
Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title_full Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title_fullStr Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title_full_unstemmed Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title_short Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources
title_sort profile of native cellulosomal proteins of clostridium cellulovorans adapted to various carbon sources
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444338/
https://www.ncbi.nlm.nih.gov/pubmed/22839966
http://dx.doi.org/10.1186/2191-0855-2-37
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