Photochemically induced ischemic stroke in rats

BACKGROUND: Photothrombosis was introduced as a model of ischemic stroke by Watson et al. in 1985. In the present paper, we describe a protocol to induce photothrombotic infarcts in rats. FINDINGS: The photosensitive dye Bengal Rose is intravenously administered and a laser beam is stereotactically...

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Autores principales: Schmidt, Antje, Hoppen, Maike, Strecker, Jan-Kolja, Diederich, Kai, Schäbitz, Wolf-Rüdiger, Schilling, Matthias, Minnerup, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444343/
https://www.ncbi.nlm.nih.gov/pubmed/22876978
http://dx.doi.org/10.1186/2040-7378-4-13
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author Schmidt, Antje
Hoppen, Maike
Strecker, Jan-Kolja
Diederich, Kai
Schäbitz, Wolf-Rüdiger
Schilling, Matthias
Minnerup, Jens
author_facet Schmidt, Antje
Hoppen, Maike
Strecker, Jan-Kolja
Diederich, Kai
Schäbitz, Wolf-Rüdiger
Schilling, Matthias
Minnerup, Jens
author_sort Schmidt, Antje
collection PubMed
description BACKGROUND: Photothrombosis was introduced as a model of ischemic stroke by Watson et al. in 1985. In the present paper, we describe a protocol to induce photothrombotic infarcts in rats. FINDINGS: The photosensitive dye Bengal Rose is intravenously administered and a laser beam is stereotactically positioned onto the skull. Illumination through the intact skull leads to local activation of Bengal Rose, which results in free radical formation, disturbance of endothelial function and thrombus formation in illuminated small cortical vessels. CONCLUSIONS: Photochemically induced infarcts cause long-term sensorimotor deficits, allow long-term survival and are particularly suitable to assess the effectiveness of neuroregenerative therapies in chronic stroke studies.
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spelling pubmed-34443432012-09-18 Photochemically induced ischemic stroke in rats Schmidt, Antje Hoppen, Maike Strecker, Jan-Kolja Diederich, Kai Schäbitz, Wolf-Rüdiger Schilling, Matthias Minnerup, Jens Exp Transl Stroke Med Short Report BACKGROUND: Photothrombosis was introduced as a model of ischemic stroke by Watson et al. in 1985. In the present paper, we describe a protocol to induce photothrombotic infarcts in rats. FINDINGS: The photosensitive dye Bengal Rose is intravenously administered and a laser beam is stereotactically positioned onto the skull. Illumination through the intact skull leads to local activation of Bengal Rose, which results in free radical formation, disturbance of endothelial function and thrombus formation in illuminated small cortical vessels. CONCLUSIONS: Photochemically induced infarcts cause long-term sensorimotor deficits, allow long-term survival and are particularly suitable to assess the effectiveness of neuroregenerative therapies in chronic stroke studies. BioMed Central 2012-08-09 /pmc/articles/PMC3444343/ /pubmed/22876978 http://dx.doi.org/10.1186/2040-7378-4-13 Text en Copyright ©2012 Schmidt et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Schmidt, Antje
Hoppen, Maike
Strecker, Jan-Kolja
Diederich, Kai
Schäbitz, Wolf-Rüdiger
Schilling, Matthias
Minnerup, Jens
Photochemically induced ischemic stroke in rats
title Photochemically induced ischemic stroke in rats
title_full Photochemically induced ischemic stroke in rats
title_fullStr Photochemically induced ischemic stroke in rats
title_full_unstemmed Photochemically induced ischemic stroke in rats
title_short Photochemically induced ischemic stroke in rats
title_sort photochemically induced ischemic stroke in rats
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444343/
https://www.ncbi.nlm.nih.gov/pubmed/22876978
http://dx.doi.org/10.1186/2040-7378-4-13
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AT schabitzwolfrudiger photochemicallyinducedischemicstrokeinrats
AT schillingmatthias photochemicallyinducedischemicstrokeinrats
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