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Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants

To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butyle...

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Autores principales: Koitabashi, Motoo, Noguchi, Masako T, Sameshima-Yamashita, Yuka, Hiradate, Syuntaro, Suzuki, Ken, Yoshida, Shigenobu, Watanabe, Takashi, Shinozaki, Yukiko, Tsushima, Seiya, Kitamoto, Hiroko K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444367/
https://www.ncbi.nlm.nih.gov/pubmed/22856640
http://dx.doi.org/10.1186/2191-0855-2-40
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author Koitabashi, Motoo
Noguchi, Masako T
Sameshima-Yamashita, Yuka
Hiradate, Syuntaro
Suzuki, Ken
Yoshida, Shigenobu
Watanabe, Takashi
Shinozaki, Yukiko
Tsushima, Seiya
Kitamoto, Hiroko K
author_facet Koitabashi, Motoo
Noguchi, Masako T
Sameshima-Yamashita, Yuka
Hiradate, Syuntaro
Suzuki, Ken
Yoshida, Shigenobu
Watanabe, Takashi
Shinozaki, Yukiko
Tsushima, Seiya
Kitamoto, Hiroko K
author_sort Koitabashi, Motoo
collection PubMed
description To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-butylene adipate) (PBSA) and poly-(butylene succinate) (PBS) films. The strain grew on the surface of soil-mounted BP films, produced breaks along the direction of hyphal growth indicated that it secreted a BP-degrading enzyme, and has directly contributing to accelerating the degradation of film. Treatment with the culture filtrate decomposed 91.2 wt%, 23.7 wt%, and 14.6 wt% of PBSA, PBS, and commercially available BP polymer blended mulch film, respectively, on unsterlized soil within 6 days. The PCR-DGGE analysis of the transition of soil microbial community during film degradation revealed that the process was accompanied with drastic changes in the population of soil fungi and Acantamoeba spp., as well as the growth of inoculated strain B47-9. It has a potential for application in the development of an effective method for accelerating degradation of used plastics under actual field conditions.
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spelling pubmed-34443672012-09-18 Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants Koitabashi, Motoo Noguchi, Masako T Sameshima-Yamashita, Yuka Hiradate, Syuntaro Suzuki, Ken Yoshida, Shigenobu Watanabe, Takashi Shinozaki, Yukiko Tsushima, Seiya Kitamoto, Hiroko K AMB Express Original Article To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-butylene adipate) (PBSA) and poly-(butylene succinate) (PBS) films. The strain grew on the surface of soil-mounted BP films, produced breaks along the direction of hyphal growth indicated that it secreted a BP-degrading enzyme, and has directly contributing to accelerating the degradation of film. Treatment with the culture filtrate decomposed 91.2 wt%, 23.7 wt%, and 14.6 wt% of PBSA, PBS, and commercially available BP polymer blended mulch film, respectively, on unsterlized soil within 6 days. The PCR-DGGE analysis of the transition of soil microbial community during film degradation revealed that the process was accompanied with drastic changes in the population of soil fungi and Acantamoeba spp., as well as the growth of inoculated strain B47-9. It has a potential for application in the development of an effective method for accelerating degradation of used plastics under actual field conditions. Springer 2012-08-02 /pmc/articles/PMC3444367/ /pubmed/22856640 http://dx.doi.org/10.1186/2191-0855-2-40 Text en Copyright ©2012 Koitabashi et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Koitabashi, Motoo
Noguchi, Masako T
Sameshima-Yamashita, Yuka
Hiradate, Syuntaro
Suzuki, Ken
Yoshida, Shigenobu
Watanabe, Takashi
Shinozaki, Yukiko
Tsushima, Seiya
Kitamoto, Hiroko K
Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title_full Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title_fullStr Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title_full_unstemmed Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title_short Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
title_sort degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444367/
https://www.ncbi.nlm.nih.gov/pubmed/22856640
http://dx.doi.org/10.1186/2191-0855-2-40
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