The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways
BACKGROUND: C-src is an evolutionarily conserved proto-oncogene that regulates cell proliferation, differentiation and apoptosis. In our previous studies, we have reported that another proto-oncogene, c-erbB(2), plays an important role in primordial follicle activation and development. We also found...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444437/ https://www.ncbi.nlm.nih.gov/pubmed/22905678 http://dx.doi.org/10.1186/1477-7827-10-58 |
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author | Du, Xiao-Yu Huang, Jian Xu, Liang-Quan Tang, Dan-Feng Wu, Lei Zhang, Li-Xia Pan, Xiao-Ling Chen, Wei-Yun Zheng, Li-Ping Zheng, Yue-Hui |
author_facet | Du, Xiao-Yu Huang, Jian Xu, Liang-Quan Tang, Dan-Feng Wu, Lei Zhang, Li-Xia Pan, Xiao-Ling Chen, Wei-Yun Zheng, Li-Ping Zheng, Yue-Hui |
author_sort | Du, Xiao-Yu |
collection | PubMed |
description | BACKGROUND: C-src is an evolutionarily conserved proto-oncogene that regulates cell proliferation, differentiation and apoptosis. In our previous studies, we have reported that another proto-oncogene, c-erbB(2), plays an important role in primordial follicle activation and development. We also found that c-src was expressed in mammalian ovaries, but its functions in primordial follicle activation remain unclear. The objective of this study is to investigate the role and mechanism of c-src during the growth of primordial follicles. METHODS: Ovaries from 2-day-old rats were cultured in vitro for 8 days. Three c-src-targeting and one negative control siRNA were designed and used in the present study. PCR, Western blotting and primordial follicle development were assessed for the silencing efficiency of the lentivirus c-src siRNA and its effect on primordial follicle onset. The expression of c-src mRNA and protein in primordial follicle growth were examined using the PCR method and immunohistochemical staining. Furthermore, the MAPK inhibitor PD98059, the PKC inhibitor Calphostin and the PI3K inhibitor LY294002 were used to explore the possible signaling pathways of c-src in primordial folliculogenesis. RESULTS: The results showed that Src protein was distributed in the ooplasmic membrane and the granulosa cell membrane in the primordial follicles, and c-src expression level increased with the growth of primordial follicle. The c-src -targeting lentivirus siRNAs had a silencing effect on c-src mRNA and protein expression. Eight days after transfection of rat ovaries with c-src siRNA, the GFP fluorescence in frozen ovarian sections was clearly discernible under a fluorescence microscope, and its relative expression level was 5-fold higher than that in the control group. Furthermore, the c-src-targeting lentivirus siRNAs lowered its relative expression level 1.96 times. We also found that the development of cultured primordial follicles was completely arrested after c-src siRNA knockdown of c-src expression. Furthermore, our studies demonstrated that folliculogenesis onset was inhibited by Calphostin, PD98059 or LY294002 treatment,but none of them down-regulated c-src expression. In contrast, the expression levels of p-PKC, p-ERK1/2 and p-PI3K in the follicles were clearly decreased by c-src siRNA transfection. Correspondingly, both Calphostin and LY294002 treatment resulted in a decrease in the p-PKC level in follicles, but no change was observed in the PD98059 group. Finally, LY294002 treatment decreased the p-PI3K expression level in the follicles, but no changes were observed in the PD98059 and Calphostin groups. CONCLUSIONS: C-src plays an important role in regulating primordial follicle activation and growth via the PI3K-PKC- ERK1/2 pathway. |
format | Online Article Text |
id | pubmed-3444437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34444372012-09-18 The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways Du, Xiao-Yu Huang, Jian Xu, Liang-Quan Tang, Dan-Feng Wu, Lei Zhang, Li-Xia Pan, Xiao-Ling Chen, Wei-Yun Zheng, Li-Ping Zheng, Yue-Hui Reprod Biol Endocrinol Research BACKGROUND: C-src is an evolutionarily conserved proto-oncogene that regulates cell proliferation, differentiation and apoptosis. In our previous studies, we have reported that another proto-oncogene, c-erbB(2), plays an important role in primordial follicle activation and development. We also found that c-src was expressed in mammalian ovaries, but its functions in primordial follicle activation remain unclear. The objective of this study is to investigate the role and mechanism of c-src during the growth of primordial follicles. METHODS: Ovaries from 2-day-old rats were cultured in vitro for 8 days. Three c-src-targeting and one negative control siRNA were designed and used in the present study. PCR, Western blotting and primordial follicle development were assessed for the silencing efficiency of the lentivirus c-src siRNA and its effect on primordial follicle onset. The expression of c-src mRNA and protein in primordial follicle growth were examined using the PCR method and immunohistochemical staining. Furthermore, the MAPK inhibitor PD98059, the PKC inhibitor Calphostin and the PI3K inhibitor LY294002 were used to explore the possible signaling pathways of c-src in primordial folliculogenesis. RESULTS: The results showed that Src protein was distributed in the ooplasmic membrane and the granulosa cell membrane in the primordial follicles, and c-src expression level increased with the growth of primordial follicle. The c-src -targeting lentivirus siRNAs had a silencing effect on c-src mRNA and protein expression. Eight days after transfection of rat ovaries with c-src siRNA, the GFP fluorescence in frozen ovarian sections was clearly discernible under a fluorescence microscope, and its relative expression level was 5-fold higher than that in the control group. Furthermore, the c-src-targeting lentivirus siRNAs lowered its relative expression level 1.96 times. We also found that the development of cultured primordial follicles was completely arrested after c-src siRNA knockdown of c-src expression. Furthermore, our studies demonstrated that folliculogenesis onset was inhibited by Calphostin, PD98059 or LY294002 treatment,but none of them down-regulated c-src expression. In contrast, the expression levels of p-PKC, p-ERK1/2 and p-PI3K in the follicles were clearly decreased by c-src siRNA transfection. Correspondingly, both Calphostin and LY294002 treatment resulted in a decrease in the p-PKC level in follicles, but no change was observed in the PD98059 group. Finally, LY294002 treatment decreased the p-PI3K expression level in the follicles, but no changes were observed in the PD98059 and Calphostin groups. CONCLUSIONS: C-src plays an important role in regulating primordial follicle activation and growth via the PI3K-PKC- ERK1/2 pathway. BioMed Central 2012-08-20 /pmc/articles/PMC3444437/ /pubmed/22905678 http://dx.doi.org/10.1186/1477-7827-10-58 Text en Copyright ©2012 Du et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Du, Xiao-Yu Huang, Jian Xu, Liang-Quan Tang, Dan-Feng Wu, Lei Zhang, Li-Xia Pan, Xiao-Ling Chen, Wei-Yun Zheng, Li-Ping Zheng, Yue-Hui The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title | The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title_full | The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title_fullStr | The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title_full_unstemmed | The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title_short | The proto-oncogene c-src is involved in primordial follicle activation through the PI3K, PKC and MAPK signaling pathways |
title_sort | proto-oncogene c-src is involved in primordial follicle activation through the pi3k, pkc and mapk signaling pathways |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444437/ https://www.ncbi.nlm.nih.gov/pubmed/22905678 http://dx.doi.org/10.1186/1477-7827-10-58 |
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