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Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity

In order to further understand the genetic basis for variation in inherent (untrained) exercise capacity, we examined the brains of 32 male rats selectively bred for high or low running capacity (HCR and LCR, respectively). The aim was to characterize the activation patterns of brain regions potenti...

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Autores principales: Foley, Teresa E., Brooks, Leah R., Gilligan, Lori J., Burghardt, Paul R., Koch, Lauren G., Britton, Steven L., Fleshner, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444461/
https://www.ncbi.nlm.nih.gov/pubmed/23028992
http://dx.doi.org/10.1371/journal.pone.0045415
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author Foley, Teresa E.
Brooks, Leah R.
Gilligan, Lori J.
Burghardt, Paul R.
Koch, Lauren G.
Britton, Steven L.
Fleshner, Monika
author_facet Foley, Teresa E.
Brooks, Leah R.
Gilligan, Lori J.
Burghardt, Paul R.
Koch, Lauren G.
Britton, Steven L.
Fleshner, Monika
author_sort Foley, Teresa E.
collection PubMed
description In order to further understand the genetic basis for variation in inherent (untrained) exercise capacity, we examined the brains of 32 male rats selectively bred for high or low running capacity (HCR and LCR, respectively). The aim was to characterize the activation patterns of brain regions potentially involved in differences in inherent running capacity between HCR and LCR. Using quantitative in situ hybridization techniques, we measured messenger ribonuclease (mRNA) levels of c-Fos, a marker of neuronal activation, in the brains of HCR and LCR rats after a single bout of acute treadmill running (7.5–15 minutes, 15° slope, 10 m/min) or after treadmill running to exhaustion (15–51 minutes, 15° slope, initial velocity 10 m/min). During verification of trait differences, HCR rats ran six times farther and three times longer prior to exhaustion than LCR rats. Running to exhaustion significantly increased c-Fos mRNA activation of several brain areas in HCR, but LCR failed to show significant elevations of c-Fos mRNA at exhaustion in the majority of areas examined compared to acutely run controls. Results from these studies suggest that there are differences in central c-Fos mRNA expression, and potential brain activation patterns, between HCR and LCR rats during treadmill running to exhaustion and these differences could be involved in the variation in inherent running capacity between lines.
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spelling pubmed-34444612012-10-01 Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity Foley, Teresa E. Brooks, Leah R. Gilligan, Lori J. Burghardt, Paul R. Koch, Lauren G. Britton, Steven L. Fleshner, Monika PLoS One Research Article In order to further understand the genetic basis for variation in inherent (untrained) exercise capacity, we examined the brains of 32 male rats selectively bred for high or low running capacity (HCR and LCR, respectively). The aim was to characterize the activation patterns of brain regions potentially involved in differences in inherent running capacity between HCR and LCR. Using quantitative in situ hybridization techniques, we measured messenger ribonuclease (mRNA) levels of c-Fos, a marker of neuronal activation, in the brains of HCR and LCR rats after a single bout of acute treadmill running (7.5–15 minutes, 15° slope, 10 m/min) or after treadmill running to exhaustion (15–51 minutes, 15° slope, initial velocity 10 m/min). During verification of trait differences, HCR rats ran six times farther and three times longer prior to exhaustion than LCR rats. Running to exhaustion significantly increased c-Fos mRNA activation of several brain areas in HCR, but LCR failed to show significant elevations of c-Fos mRNA at exhaustion in the majority of areas examined compared to acutely run controls. Results from these studies suggest that there are differences in central c-Fos mRNA expression, and potential brain activation patterns, between HCR and LCR rats during treadmill running to exhaustion and these differences could be involved in the variation in inherent running capacity between lines. Public Library of Science 2012-09-17 /pmc/articles/PMC3444461/ /pubmed/23028992 http://dx.doi.org/10.1371/journal.pone.0045415 Text en © 2012 Foley et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Foley, Teresa E.
Brooks, Leah R.
Gilligan, Lori J.
Burghardt, Paul R.
Koch, Lauren G.
Britton, Steven L.
Fleshner, Monika
Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title_full Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title_fullStr Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title_full_unstemmed Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title_short Brain Activation Patterns at Exhaustion in Rats That Differ in Inherent Exercise Capacity
title_sort brain activation patterns at exhaustion in rats that differ in inherent exercise capacity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444461/
https://www.ncbi.nlm.nih.gov/pubmed/23028992
http://dx.doi.org/10.1371/journal.pone.0045415
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