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Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field
The effect of coupling the salt concentration gradient crystallization method with the use of the paramagnetic salt MnCl(2) and a magnetic field is reported. The use of a simple magnetic device is proposed to have a significant effect on hen egg-white lysozyme crystal growth. Large single crystals g...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445808/ https://www.ncbi.nlm.nih.gov/pubmed/22997475 http://dx.doi.org/10.1107/S0021889812036060 |
| Sumario: | The effect of coupling the salt concentration gradient crystallization method with the use of the paramagnetic salt MnCl(2) and a magnetic field is reported. The use of a simple magnetic device is proposed to have a significant effect on hen egg-white lysozyme crystal growth. Large single crystals greater than 10 mm(3) in volume with optical perfection were consistently obtained in this study. |
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