Cargando…
Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field
The effect of coupling the salt concentration gradient crystallization method with the use of the paramagnetic salt MnCl(2) and a magnetic field is reported. The use of a simple magnetic device is proposed to have a significant effect on hen egg-white lysozyme crystal growth. Large single crystals g...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445808/ https://www.ncbi.nlm.nih.gov/pubmed/22997475 http://dx.doi.org/10.1107/S0021889812036060 |
_version_ | 1782243862901686272 |
---|---|
author | Magay, Elena Cho, Sang Jin Kim, Shin Ae |
author_facet | Magay, Elena Cho, Sang Jin Kim, Shin Ae |
author_sort | Magay, Elena |
collection | PubMed |
description | The effect of coupling the salt concentration gradient crystallization method with the use of the paramagnetic salt MnCl(2) and a magnetic field is reported. The use of a simple magnetic device is proposed to have a significant effect on hen egg-white lysozyme crystal growth. Large single crystals greater than 10 mm(3) in volume with optical perfection were consistently obtained in this study. |
format | Online Article Text |
id | pubmed-3445808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-34458082012-09-20 Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field Magay, Elena Cho, Sang Jin Kim, Shin Ae J Appl Crystallogr Laboratory Notes The effect of coupling the salt concentration gradient crystallization method with the use of the paramagnetic salt MnCl(2) and a magnetic field is reported. The use of a simple magnetic device is proposed to have a significant effect on hen egg-white lysozyme crystal growth. Large single crystals greater than 10 mm(3) in volume with optical perfection were consistently obtained in this study. International Union of Crystallography 2012-10-01 2012-09-13 /pmc/articles/PMC3445808/ /pubmed/22997475 http://dx.doi.org/10.1107/S0021889812036060 Text en © Magay, Cho and Kim 2012 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Laboratory Notes Magay, Elena Cho, Sang Jin Kim, Shin Ae Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title | Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title_full | Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title_fullStr | Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title_full_unstemmed | Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title_short | Enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
title_sort | enhancing the volume and the optical quality of hen egg-white lysozyme crystals by coupling the salt concentration gradient crystallization method with a magnetic field |
topic | Laboratory Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445808/ https://www.ncbi.nlm.nih.gov/pubmed/22997475 http://dx.doi.org/10.1107/S0021889812036060 |
work_keys_str_mv | AT magayelena enhancingthevolumeandtheopticalqualityofheneggwhitelysozymecrystalsbycouplingthesaltconcentrationgradientcrystallizationmethodwithamagneticfield AT chosangjin enhancingthevolumeandtheopticalqualityofheneggwhitelysozymecrystalsbycouplingthesaltconcentrationgradientcrystallizationmethodwithamagneticfield AT kimshinae enhancingthevolumeandtheopticalqualityofheneggwhitelysozymecrystalsbycouplingthesaltconcentrationgradientcrystallizationmethodwithamagneticfield |