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Schwann cells migrate along axons in the absence of GDNF signaling

BACKGROUND: During development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the integrity of myelinated as well as nonmyelinated axons. To provide adequate support func...

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Autores principales: Heermann, Stephan, Spittau, Björn, Zajzon, Katalin, Schwab, Markus H, Krieglstein, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445819/
https://www.ncbi.nlm.nih.gov/pubmed/22863354
http://dx.doi.org/10.1186/1471-2202-13-92
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author Heermann, Stephan
Spittau, Björn
Zajzon, Katalin
Schwab, Markus H
Krieglstein, Kerstin
author_facet Heermann, Stephan
Spittau, Björn
Zajzon, Katalin
Schwab, Markus H
Krieglstein, Kerstin
author_sort Heermann, Stephan
collection PubMed
description BACKGROUND: During development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the integrity of myelinated as well as nonmyelinated axons. To provide adequate support functions, SC colonize entire nerves. One important prerequisite for this is their migration into distal axonal regions. RESULTS: Here, we studied the role of Glial cell line derived neurotrophic factor (GDNF), a TGF-beta related growth factor, for SC migration. To this end we used a superior cervical ganglion (SCG) explant-SC migration assay, GDNF null mutant mouse embryos and a chemical inhibitor for GDNF signaling in combination with time-lapse imaging. We found that GDNF signaling is dispensable for SC migration along murine embryonic sympathetic axons. Furthermore, in vivo analyzes revealed that SC migration along the sciatic nerve is also not dependent on GDNF. CONCLUSIONS: In contrast to previous in vitro findings in the sciatic nerve and a SC precursor cell line, our results clearly indicate that GDNF is dispensable for embryonic SC migration. This is demonstrated for the sympathetic nervous system and also for the sciatic nerve in mouse.
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spelling pubmed-34458192012-09-20 Schwann cells migrate along axons in the absence of GDNF signaling Heermann, Stephan Spittau, Björn Zajzon, Katalin Schwab, Markus H Krieglstein, Kerstin BMC Neurosci Research Article BACKGROUND: During development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the integrity of myelinated as well as nonmyelinated axons. To provide adequate support functions, SC colonize entire nerves. One important prerequisite for this is their migration into distal axonal regions. RESULTS: Here, we studied the role of Glial cell line derived neurotrophic factor (GDNF), a TGF-beta related growth factor, for SC migration. To this end we used a superior cervical ganglion (SCG) explant-SC migration assay, GDNF null mutant mouse embryos and a chemical inhibitor for GDNF signaling in combination with time-lapse imaging. We found that GDNF signaling is dispensable for SC migration along murine embryonic sympathetic axons. Furthermore, in vivo analyzes revealed that SC migration along the sciatic nerve is also not dependent on GDNF. CONCLUSIONS: In contrast to previous in vitro findings in the sciatic nerve and a SC precursor cell line, our results clearly indicate that GDNF is dispensable for embryonic SC migration. This is demonstrated for the sympathetic nervous system and also for the sciatic nerve in mouse. BioMed Central 2012-08-03 /pmc/articles/PMC3445819/ /pubmed/22863354 http://dx.doi.org/10.1186/1471-2202-13-92 Text en Copyright ©2012 Heermann et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Heermann, Stephan
Spittau, Björn
Zajzon, Katalin
Schwab, Markus H
Krieglstein, Kerstin
Schwann cells migrate along axons in the absence of GDNF signaling
title Schwann cells migrate along axons in the absence of GDNF signaling
title_full Schwann cells migrate along axons in the absence of GDNF signaling
title_fullStr Schwann cells migrate along axons in the absence of GDNF signaling
title_full_unstemmed Schwann cells migrate along axons in the absence of GDNF signaling
title_short Schwann cells migrate along axons in the absence of GDNF signaling
title_sort schwann cells migrate along axons in the absence of gdnf signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445819/
https://www.ncbi.nlm.nih.gov/pubmed/22863354
http://dx.doi.org/10.1186/1471-2202-13-92
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