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A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level

We present a novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers. It is based on transcription factors and we used it to isolate new L-lysine producing mutants of...

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Detalles Bibliográficos
Autores principales:	Binder, Stephan, Schendzielorz, Georg, Stäbler, Norma, Krumbach, Karin, Hoffmann, Kristina, Bott, Michael, Eggeling, Lothar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446293/ https://www.ncbi.nlm.nih.gov/pubmed/22640862 http://dx.doi.org/10.1186/gb-2012-13-5-r40

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