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Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators?
INTRODUCTION: We have taken advantage of the large screening capacity of a multiplex immunoassay to better define the respective contribution of articular versus systemic cytokines in experimental arthritis. METHODS: We performed a follow up (from 7 hours to 14 days) multiplex analysis of 24 cytokin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446427/ https://www.ncbi.nlm.nih.gov/pubmed/22414623 http://dx.doi.org/10.1186/ar3774 |
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author | Paquet, Joseph Goebel, Jean-Christophe Delaunay, Camille Pinzano, Astrid Grossin, Laurent Cournil-Henrionnet, Christel Gillet, Pierre Netter, Patrick Jouzeau, Jean-Yves Moulin, David |
author_facet | Paquet, Joseph Goebel, Jean-Christophe Delaunay, Camille Pinzano, Astrid Grossin, Laurent Cournil-Henrionnet, Christel Gillet, Pierre Netter, Patrick Jouzeau, Jean-Yves Moulin, David |
author_sort | Paquet, Joseph |
collection | PubMed |
description | INTRODUCTION: We have taken advantage of the large screening capacity of a multiplex immunoassay to better define the respective contribution of articular versus systemic cytokines in experimental arthritis. METHODS: We performed a follow up (from 7 hours to 14 days) multiplex analysis of 24 cytokines in synovial fluid and sera of rats developing Antigen-Induced Arthritis (AIA) and confronted their protein level changes with molecular, biochemical, histological and clinical events occurring in the course of the disease. RESULTS: The time-scheduled findings in arthritic joints correlated with time-dependent changes of cytokine amounts in joint effusions but not with their blood levels. From seven hours after sensitization, high levels of chemokines (MCP-1, MIP1α, GRO/KC, RANTES, eotaxin) were found in synovial fluid of arthritic knees whereas perivascular infiltration occurred in the synovium; local release of inflammatory cytokines (IFNγ, IL-1β, IL-6) preceded the spreading of inflammation and resulted in progressive degradation of cartilage and bone. Finally a local overexpression of several cytokines/adipocytokines poorly described in arthritis (IL-13, IL-18, leptin) was observed. CONCLUSIONS: Distinct panels of cytokines were found in arthritic fluid during AIA, and the expected effect of mediators correlated well with changes occurring in joint tissues. Moreover, multiplex analysis could be helpful to identify new pathogenic mediators and to elucidate the mechanisms supporting the efficacy of putative targeted therapies. |
format | Online Article Text |
id | pubmed-3446427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34464272012-09-20 Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? Paquet, Joseph Goebel, Jean-Christophe Delaunay, Camille Pinzano, Astrid Grossin, Laurent Cournil-Henrionnet, Christel Gillet, Pierre Netter, Patrick Jouzeau, Jean-Yves Moulin, David Arthritis Res Ther Research Article INTRODUCTION: We have taken advantage of the large screening capacity of a multiplex immunoassay to better define the respective contribution of articular versus systemic cytokines in experimental arthritis. METHODS: We performed a follow up (from 7 hours to 14 days) multiplex analysis of 24 cytokines in synovial fluid and sera of rats developing Antigen-Induced Arthritis (AIA) and confronted their protein level changes with molecular, biochemical, histological and clinical events occurring in the course of the disease. RESULTS: The time-scheduled findings in arthritic joints correlated with time-dependent changes of cytokine amounts in joint effusions but not with their blood levels. From seven hours after sensitization, high levels of chemokines (MCP-1, MIP1α, GRO/KC, RANTES, eotaxin) were found in synovial fluid of arthritic knees whereas perivascular infiltration occurred in the synovium; local release of inflammatory cytokines (IFNγ, IL-1β, IL-6) preceded the spreading of inflammation and resulted in progressive degradation of cartilage and bone. Finally a local overexpression of several cytokines/adipocytokines poorly described in arthritis (IL-13, IL-18, leptin) was observed. CONCLUSIONS: Distinct panels of cytokines were found in arthritic fluid during AIA, and the expected effect of mediators correlated well with changes occurring in joint tissues. Moreover, multiplex analysis could be helpful to identify new pathogenic mediators and to elucidate the mechanisms supporting the efficacy of putative targeted therapies. BioMed Central 2012 2012-03-13 /pmc/articles/PMC3446427/ /pubmed/22414623 http://dx.doi.org/10.1186/ar3774 Text en Copyright ©2011 Paquet et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Paquet, Joseph Goebel, Jean-Christophe Delaunay, Camille Pinzano, Astrid Grossin, Laurent Cournil-Henrionnet, Christel Gillet, Pierre Netter, Patrick Jouzeau, Jean-Yves Moulin, David Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title | Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title_full | Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title_fullStr | Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title_full_unstemmed | Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title_short | Cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
title_sort | cytokines profiling by multiplex analysis in experimental arthritis: which pathophysiological relevance for articular versus systemic mediators? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446427/ https://www.ncbi.nlm.nih.gov/pubmed/22414623 http://dx.doi.org/10.1186/ar3774 |
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