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The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication

Equine herpesvirus 4 (EHV-4) is an important equine pathogen that causes respiratory tract disease among horses worldwide. Glycoprotein K (gK) homologues have been identified in several alphaherpesviruses as a major player in virus entry, replication, and spread. In the present study, EHV-4 gK-delet...

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Autores principales: Azab, Walid, El-Sheikh, Abuelyazeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446760/
https://www.ncbi.nlm.nih.gov/pubmed/23012623
http://dx.doi.org/10.3390/v4081258
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author Azab, Walid
El-Sheikh, Abuelyazeed
author_facet Azab, Walid
El-Sheikh, Abuelyazeed
author_sort Azab, Walid
collection PubMed
description Equine herpesvirus 4 (EHV-4) is an important equine pathogen that causes respiratory tract disease among horses worldwide. Glycoprotein K (gK) homologues have been identified in several alphaherpesviruses as a major player in virus entry, replication, and spread. In the present study, EHV-4 gK-deletion mutant has been generated by using bacterial artificial chromosome technology and Red mutagenesis to investigate the role of gK in EHV-4 replication. Our findings reported here show that gK is essential for virus replication in vitro and that the gK-negative strain was not able to be reconstituted in equine cells. It is noteworthy that these findings agree with the previously published study describing gK deletion in other alphaherpesviruses.
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spelling pubmed-34467602012-09-25 The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication Azab, Walid El-Sheikh, Abuelyazeed Viruses Communication Equine herpesvirus 4 (EHV-4) is an important equine pathogen that causes respiratory tract disease among horses worldwide. Glycoprotein K (gK) homologues have been identified in several alphaherpesviruses as a major player in virus entry, replication, and spread. In the present study, EHV-4 gK-deletion mutant has been generated by using bacterial artificial chromosome technology and Red mutagenesis to investigate the role of gK in EHV-4 replication. Our findings reported here show that gK is essential for virus replication in vitro and that the gK-negative strain was not able to be reconstituted in equine cells. It is noteworthy that these findings agree with the previously published study describing gK deletion in other alphaherpesviruses. MDPI 2012-08-07 /pmc/articles/PMC3446760/ /pubmed/23012623 http://dx.doi.org/10.3390/v4081258 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Azab, Walid
El-Sheikh, Abuelyazeed
The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title_full The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title_fullStr The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title_full_unstemmed The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title_short The Role of Equine Herpesvirus Type 4 Glycoprotein K in Virus Replication
title_sort role of equine herpesvirus type 4 glycoprotein k in virus replication
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446760/
https://www.ncbi.nlm.nih.gov/pubmed/23012623
http://dx.doi.org/10.3390/v4081258
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