A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridi...

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Detalles Bibliográficos
Autores principales: Hong, Guolin, Liu, Yinhuan, Chen, Wei, Weng, Shaohuang, Liu, Qicai, Liu, Ailin, Zheng, Daoxin, Lin, Xinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446862/
https://www.ncbi.nlm.nih.gov/pubmed/23028223
http://dx.doi.org/10.2147/IJN.S35177
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author Hong, Guolin
Liu, Yinhuan
Chen, Wei
Weng, Shaohuang
Liu, Qicai
Liu, Ailin
Zheng, Daoxin
Lin, Xinhua
author_facet Hong, Guolin
Liu, Yinhuan
Chen, Wei
Weng, Shaohuang
Liu, Qicai
Liu, Ailin
Zheng, Daoxin
Lin, Xinhua
author_sort Hong, Guolin
collection PubMed
description A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.
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spelling pubmed-34468622012-10-01 A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method Hong, Guolin Liu, Yinhuan Chen, Wei Weng, Shaohuang Liu, Qicai Liu, Ailin Zheng, Daoxin Lin, Xinhua Int J Nanomedicine Original Research A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection. Dove Medical Press 2012 2012-09-14 /pmc/articles/PMC3446862/ /pubmed/23028223 http://dx.doi.org/10.2147/IJN.S35177 Text en © 2012 Hong et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Hong, Guolin
Liu, Yinhuan
Chen, Wei
Weng, Shaohuang
Liu, Qicai
Liu, Ailin
Zheng, Daoxin
Lin, Xinhua
A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_full A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_fullStr A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_full_unstemmed A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_short A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_sort sandwich-type dna electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3446862/
https://www.ncbi.nlm.nih.gov/pubmed/23028223
http://dx.doi.org/10.2147/IJN.S35177
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