Cargando…
Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig
A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the chemical coding patterns of these neurons during porcine proliferative enteropathy (PE). To answer this question, juve...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Humana Press Inc
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447145/ https://www.ncbi.nlm.nih.gov/pubmed/22170039 http://dx.doi.org/10.1007/s12031-011-9691-3 |
_version_ | 1782244064010174464 |
---|---|
author | Wojtkiewicz, Joanna Równiak, Maciej Gonkowski, Sławomir Crayton, Robert Majewski, Mariusz Robak, Anna Białkowska, Joanna Barczewska, Monika |
author_facet | Wojtkiewicz, Joanna Równiak, Maciej Gonkowski, Sławomir Crayton, Robert Majewski, Mariusz Robak, Anna Białkowska, Joanna Barczewska, Monika |
author_sort | Wojtkiewicz, Joanna |
collection | PubMed |
description | A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the chemical coding patterns of these neurons during porcine proliferative enteropathy (PE). To answer this question, juvenile Large White Polish pigs with clinically diagnosed Lawsonia intracellularis infection (PE; n = 3) and a group of uninfected controls (C; n = 3) were compared. The retrograde tracer fast blue (FB) was injected into the descending colons of all animals and then tissue comprising IMGs from both groups was processed for double-labeling immunofluorescence with calbindin-D28k (CB) in combination with either tyrosine hydroxylase (TH), neuropeptide Y (NPY), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), nitric oxide synthase, Leu-enkephalin, substance P, vesicular acetylcholine transporter, galanin, or pituitary adenylate cyclase-activating polypeptide. Immunohistochemistry revealed changes in the chemical coding pattern of calbindin-immunoreactive neurons in the inferior mesenteric ganglia of the pig. In control animals, FB/CB-positive neurons were immunoreactive to TH, NPY, SOM, and VIP. In the experimental group, TH-expressing neurons were unaffected, NPY-expressing neurons were increased, whereas the number of neurons immunoreactive to SOM or VIP was reduced. Changes in chemical coding of CB neurons during PE may play an important role in adaptation of these IMG cells under pathological conditions. |
format | Online Article Text |
id | pubmed-3447145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Humana Press Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-34471452012-09-27 Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig Wojtkiewicz, Joanna Równiak, Maciej Gonkowski, Sławomir Crayton, Robert Majewski, Mariusz Robak, Anna Białkowska, Joanna Barczewska, Monika J Mol Neurosci Article A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the chemical coding patterns of these neurons during porcine proliferative enteropathy (PE). To answer this question, juvenile Large White Polish pigs with clinically diagnosed Lawsonia intracellularis infection (PE; n = 3) and a group of uninfected controls (C; n = 3) were compared. The retrograde tracer fast blue (FB) was injected into the descending colons of all animals and then tissue comprising IMGs from both groups was processed for double-labeling immunofluorescence with calbindin-D28k (CB) in combination with either tyrosine hydroxylase (TH), neuropeptide Y (NPY), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), nitric oxide synthase, Leu-enkephalin, substance P, vesicular acetylcholine transporter, galanin, or pituitary adenylate cyclase-activating polypeptide. Immunohistochemistry revealed changes in the chemical coding pattern of calbindin-immunoreactive neurons in the inferior mesenteric ganglia of the pig. In control animals, FB/CB-positive neurons were immunoreactive to TH, NPY, SOM, and VIP. In the experimental group, TH-expressing neurons were unaffected, NPY-expressing neurons were increased, whereas the number of neurons immunoreactive to SOM or VIP was reduced. Changes in chemical coding of CB neurons during PE may play an important role in adaptation of these IMG cells under pathological conditions. Humana Press Inc 2011-12-15 2012 /pmc/articles/PMC3447145/ /pubmed/22170039 http://dx.doi.org/10.1007/s12031-011-9691-3 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Wojtkiewicz, Joanna Równiak, Maciej Gonkowski, Sławomir Crayton, Robert Majewski, Mariusz Robak, Anna Białkowska, Joanna Barczewska, Monika Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title | Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title_full | Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title_fullStr | Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title_full_unstemmed | Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title_short | Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig |
title_sort | proliferative enteropathy (pe)—induced changes in the calbindin-immunoreactive (cb-ir) neurons of inferior mesenteric ganglion supplying the descending colon in the pig |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447145/ https://www.ncbi.nlm.nih.gov/pubmed/22170039 http://dx.doi.org/10.1007/s12031-011-9691-3 |
work_keys_str_mv | AT wojtkiewiczjoanna proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT rowniakmaciej proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT gonkowskisławomir proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT craytonrobert proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT majewskimariusz proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT robakanna proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT białkowskajoanna proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig AT barczewskamonika proliferativeenteropathypeinducedchangesinthecalbindinimmunoreactivecbirneuronsofinferiormesentericganglionsupplyingthedescendingcoloninthepig |