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An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

A sequential protocol of multidimensional fractionation was optimised to enable the comparative profiling of fractions of proteomes from cultured human cells. Differential detergent fractionation was employed as a first step to obtain fractions enriched for cytosolic, membrane/organelle, nuclear, an...

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Detalles Bibliográficos
Autores principales:	Tooth, David John, Gopala Krishna, Varun, Layfield, Robert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447371/ https://www.ncbi.nlm.nih.gov/pubmed/23008771 http://dx.doi.org/10.1155/2012/735132

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