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Cell death detection by quantitative three-dimensional single-cell tomography

Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties o...

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Detalles Bibliográficos
Autores principales: Cheng, Nai-Chia, Hsieh, Tsung-Hsun, Wang, Yu-Ta, Lai, Chien-Chih, Chang, Chia-Kai, Lin, Ming-Yi, Huang, Ding-Wei, Tjiu, Jeng-Wei, Huang, Sheng-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447553/
https://www.ncbi.nlm.nih.gov/pubmed/23024905
http://dx.doi.org/10.1364/BOE.3.002111
Descripción
Sumario:Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level.