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Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis

BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 1...

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Autores principales: Rødland, Ernst Kristian, Ueland, Thor, Bjørnsen, Stine, Sagen, Ellen Lund, Dahl, Christen Peder, Naalsund, Anne, Mollnes, Tom Eirik, Brosstad, Frank R, Müller, Fredrik, Aukrust, Pål, Frøland, Stig S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447666/
https://www.ncbi.nlm.nih.gov/pubmed/22731696
http://dx.doi.org/10.1186/1471-2334-12-144
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author Rødland, Ernst Kristian
Ueland, Thor
Bjørnsen, Stine
Sagen, Ellen Lund
Dahl, Christen Peder
Naalsund, Anne
Mollnes, Tom Eirik
Brosstad, Frank R
Müller, Fredrik
Aukrust, Pål
Frøland, Stig S
author_facet Rødland, Ernst Kristian
Ueland, Thor
Bjørnsen, Stine
Sagen, Ellen Lund
Dahl, Christen Peder
Naalsund, Anne
Mollnes, Tom Eirik
Brosstad, Frank R
Müller, Fredrik
Aukrust, Pål
Frøland, Stig S
author_sort Rødland, Ernst Kristian
collection PubMed
description BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR. RESULTS: Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation. CONCLUSIONS: The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA.
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spelling pubmed-34476662012-09-21 Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis Rødland, Ernst Kristian Ueland, Thor Bjørnsen, Stine Sagen, Ellen Lund Dahl, Christen Peder Naalsund, Anne Mollnes, Tom Eirik Brosstad, Frank R Müller, Fredrik Aukrust, Pål Frøland, Stig S BMC Infect Dis Research Article BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR. RESULTS: Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation. CONCLUSIONS: The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA. BioMed Central 2012-06-25 /pmc/articles/PMC3447666/ /pubmed/22731696 http://dx.doi.org/10.1186/1471-2334-12-144 Text en Copyright ©2012 Rødland et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rødland, Ernst Kristian
Ueland, Thor
Bjørnsen, Stine
Sagen, Ellen Lund
Dahl, Christen Peder
Naalsund, Anne
Mollnes, Tom Eirik
Brosstad, Frank R
Müller, Fredrik
Aukrust, Pål
Frøland, Stig S
Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title_full Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title_fullStr Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title_full_unstemmed Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title_short Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
title_sort systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447666/
https://www.ncbi.nlm.nih.gov/pubmed/22731696
http://dx.doi.org/10.1186/1471-2334-12-144
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