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Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis
BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 1...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447666/ https://www.ncbi.nlm.nih.gov/pubmed/22731696 http://dx.doi.org/10.1186/1471-2334-12-144 |
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author | Rødland, Ernst Kristian Ueland, Thor Bjørnsen, Stine Sagen, Ellen Lund Dahl, Christen Peder Naalsund, Anne Mollnes, Tom Eirik Brosstad, Frank R Müller, Fredrik Aukrust, Pål Frøland, Stig S |
author_facet | Rødland, Ernst Kristian Ueland, Thor Bjørnsen, Stine Sagen, Ellen Lund Dahl, Christen Peder Naalsund, Anne Mollnes, Tom Eirik Brosstad, Frank R Müller, Fredrik Aukrust, Pål Frøland, Stig S |
author_sort | Rødland, Ernst Kristian |
collection | PubMed |
description | BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR. RESULTS: Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation. CONCLUSIONS: The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA. |
format | Online Article Text |
id | pubmed-3447666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34476662012-09-21 Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis Rødland, Ernst Kristian Ueland, Thor Bjørnsen, Stine Sagen, Ellen Lund Dahl, Christen Peder Naalsund, Anne Mollnes, Tom Eirik Brosstad, Frank R Müller, Fredrik Aukrust, Pål Frøland, Stig S BMC Infect Dis Research Article BACKGROUND: The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species. METHODS: Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR. RESULTS: Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation. CONCLUSIONS: The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA. BioMed Central 2012-06-25 /pmc/articles/PMC3447666/ /pubmed/22731696 http://dx.doi.org/10.1186/1471-2334-12-144 Text en Copyright ©2012 Rødland et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rødland, Ernst Kristian Ueland, Thor Bjørnsen, Stine Sagen, Ellen Lund Dahl, Christen Peder Naalsund, Anne Mollnes, Tom Eirik Brosstad, Frank R Müller, Fredrik Aukrust, Pål Frøland, Stig S Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title_full | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title_fullStr | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title_full_unstemmed | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title_short | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
title_sort | systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447666/ https://www.ncbi.nlm.nih.gov/pubmed/22731696 http://dx.doi.org/10.1186/1471-2334-12-144 |
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