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Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag

BACKGROUND: Receptors with a single transmembrane (TM) domain are essential for the signal transduction across the cell membrane. NMR spectroscopy is a powerful tool to study structure of the single TM domain. The expression and purification of a TM domain in Escherichia coli (E.coli) is challenging...

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Detalles Bibliográficos
Autores principales:	Li, Qingxin, Chen, Angela Shuyi, Gayen, Shovanlal, Kang, CongBao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447675/ https://www.ncbi.nlm.nih.gov/pubmed/22510322 http://dx.doi.org/10.1186/1475-2859-11-45

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